Light and electron microscopic examination of exocrine pancreas using zinc iodide-osmium tetroxide technique

Okajimas Folia Anat Jpn. 1998 Dec;75(5):247-50. doi: 10.2535/ofaj1936.75.5_247.

Abstract

Zinc iodide-osmium tetroxide (ZIO) fixation/staining technique is a metallophilic technique which has been used for the examination of various tissues and cell types. We examined the ZIO (+) cell types in rat exocrine pancreas to obtain further evidence for the significance of the reaction. Among mostly non-reactive pancreatic acinar cells there were ZIO (+) acinar cells of varying staining intensity. Zymogenic granules and centroacinar cells were completely non-reactive. Our electron microscopic findings support the view that the reactivity of the technique used is cell specific but not cell type or organelle specific.

MeSH terms

  • Animals
  • Cytoplasmic Granules / ultrastructure
  • Fixatives
  • Iodides* / chemistry
  • Microscopy, Electron / methods
  • Osmium Tetroxide* / chemistry
  • Pancreas / cytology*
  • Rats
  • Rats, Wistar
  • Staining and Labeling / methods
  • Tissue Fixation / methods*
  • Zinc Compounds* / chemistry

Substances

  • Fixatives
  • Iodides
  • Zinc Compounds
  • zinc iodide
  • Osmium Tetroxide