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Hypoxia inducible factors regulate pluripotency and proliferation in human embryonic stem cells cultured at reduced oxygen tensions.
Forristal CE, Wright KL, Hanley NA, Oreffo RO, Houghton FD. Forristal CE, et al. Reproduction. 2010 Jan;139(1):85-97. doi: 10.1530/REP-09-0300. Reproduction. 2010. PMID: 19755485 Free PMC article.
Human embryonic stem (hES) cells are routinely cultured under atmospheric, 20% oxygen tensions but are derived from embryos which reside in a 3-5% oxygen (hypoxic) environment. ...This study investigates the importance of sp
Human embryonic stem (hES) cells are routinely cultured under atmospheric, 20% oxygen tensions
Environmental oxygen tension regulates the energy metabolism and self-renewal of human embryonic stem cells.
Forristal CE, Christensen DR, Chinnery FE, Petruzzelli R, Parry KL, Sanchez-Elsner T, Houghton FD. Forristal CE, et al. PLoS One. 2013 May 6;8(5):e62507. doi: 10.1371/journal.pone.0062507. Print 2013. PLoS One. 2013. PMID: 23671606 Free PMC article.
Energy metabolism is intrinsic to cell viability but surprisingly has been little studied in human embryonic stem cells (hESCs). ...GLUT1 expression correlated with glucose consumption and using siRNA and chromatin immunoprecipitation was found …
Energy metabolism is intrinsic to cell viability but surprisingly has been little studied in human embryonic stem