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Bioengineering the ovarian follicle microenvironment.
Shea LD, Woodruff TK, Shikanov A. Shea LD, et al. Annu Rev Biomed Eng. 2014 Jul 11;16:29-52. doi: 10.1146/annurev-bioeng-071813-105131. Epub 2014 May 14. Annu Rev Biomed Eng. 2014. PMID: 24849592 Free PMC article. Review.
Clinically established fertility preservation methods, such as egg and embryo cryopreservation, are not applicable to all patients, which has motivated the development of strategies that involve ovarian tissue removal and cryopreservation before the first sterilizing treat …
Clinically established fertility preservation methods, such as egg and embryo cryopreservation, are not applicable to all patients, which ha …
Engineering the follicle microenvironment.
West ER, Shea LD, Woodruff TK. West ER, et al. Among authors: shea ld. Semin Reprod Med. 2007 Jul;25(4):287-99. doi: 10.1055/s-2007-980222. Semin Reprod Med. 2007. PMID: 17594609 Free PMC article.
In vitro ovarian follicle culture provides a tool to investigate folliculogenesis, and may one day provide women with fertility-preservation options. The application of tissue engineering principles to ovarian follicle maturation may enable the …
In vitro ovarian follicle culture provides a tool to investigate folliculogenesis, and may one day provide women with fertilit …
Synergy of Paracrine Signaling During Early-Stage Mouse Ovarian Follicle Development In Vitro.
Zhou H, Decker JT, Lemke MM, Tomaszweski CE, Shea LD, Arnold KB, Shikanov A. Zhou H, et al. Among authors: shea ld. Cell Mol Bioeng. 2018 Aug 9;11(5):435-450. doi: 10.1007/s12195-018-0545-8. eCollection 2018 Oct. Cell Mol Bioeng. 2018. PMID: 31719893 Free PMC article.
INTRODUCTION: Paracrine signals, such as soluble cytokines and extracellular matrix cues, are essential for the survival and development of multicellular ovarian follicles. While it is well established that hydrogel-based culture systems successfully support the growth of …
INTRODUCTION: Paracrine signals, such as soluble cytokines and extracellular matrix cues, are essential for the survival and development of …
Hydrogel network design using multifunctional macromers to coordinate tissue maturation in ovarian follicle culture.
Shikanov A, Smith RM, Xu M, Woodruff TK, Shea LD. Shikanov A, et al. Among authors: shea ld. Biomaterials. 2011 Apr;32(10):2524-31. doi: 10.1016/j.biomaterials.2010.12.027. Epub 2011 Jan 17. Biomaterials. 2011. PMID: 21247629 Free PMC article.
For trifunctional crosslinkers, the hydrogels supported a 17-fold volumetric expansion of the tissue during culture, with expansion dependent on the ability of the follicle to rearrange its microenvironment, which is controlled through the sensitivity of the cross-l …
For trifunctional crosslinkers, the hydrogels supported a 17-fold volumetric expansion of the tissue during culture, with expansion dependen …