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Characterization of the fertilization antigen 1 for the development of a contraceptive vaccine.
Naz RK, Phillips TM, Rosenblum BB. Naz RK, et al. Proc Natl Acad Sci U S A. 1986 Aug;83(15):5713-7. doi: 10.1073/pnas.83.15.5713. Proc Natl Acad Sci U S A. 1986. PMID: 3461457 Free PMC article.
Following removal of the detergent and extensive dialysis at pH 5.8 or treatment with 0.15 M NaCl, even in the presence of detergent, a monomer of 23 kDa was detected. Two-dimensional PAGE of FA-1 showed, four or five polypeptides in the 47-kDa or 23-kDa range. ...
Following removal of the detergent and extensive dialysis at pH 5.8 or treatment with 0.15 M NaCl, even in the presence of detergent, …