Antioxidant and antigenotoxic potential of Ramaria largentii Marr & D. E. Stuntz, a wild edible mushroom collected from Northeast Romania

Food Chem Toxicol. 2017 Oct;108(Pt B):429-437. doi: 10.1016/j.fct.2017.02.006. Epub 2017 Feb 5.

Abstract

Ramaria largentii Marr & D. E. Stuntz (orange coral mushroom) is a wild edible mushroom whose chemical composition and bioactivity have not been investigated. Herein, we present a study on the phenolic constituents, antioxidant and antigenotoxic effects of a hydromethanolic extract of the fruiting bodies. Total phenolic content, estimated by Folin-Ciocalteu assay, was found to be 42.33 ± 0.18 mg GAE/g. Protocatechuic and vanillic acids were detected by HPLC-DAD-ESI-MS. The extract showed good free radical scavenging and reducing capacities (EC50 = 64.3 ± 0.2 and 61.54 ± 0.46 μg/mL, respectively). In normal Vero cells, the extract (100, 200 and 300 μg/mL) showed no genotoxic potential and moreover, almost completely protected DNA against H2O2-induced damage (2.09-7.91% tail DNA) (24 and 48 h pre-treatment). Taken together, the results of our study show that Ramaria largentii extract is devoid of genotoxicity and has a remarkable DNA protective activity against H2O2-induced damage.

Keywords: Antioxidant activity; Genoprotection; Hydrogen peroxide; Mushrooms; Oxidative stress-induced DNA damage; Phenolics.

MeSH terms

  • Agaricales / chemistry*
  • Animals
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Arachidonate 15-Lipoxygenase / metabolism
  • Cell Survival
  • Chelating Agents
  • Chlorocebus aethiops
  • Food Analysis
  • Iron
  • Lipoxygenase Inhibitors / chemistry
  • Lipoxygenase Inhibitors / pharmacology
  • Mutagenicity Tests
  • Oxidation-Reduction
  • Phenols / chemistry
  • Phenols / pharmacology
  • Romania
  • Vero Cells

Substances

  • Antioxidants
  • Chelating Agents
  • Lipoxygenase Inhibitors
  • Phenols
  • Iron
  • Arachidonate 15-Lipoxygenase