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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols.
Apraiz A, Mitxelena J, Zubiaga A. Apraiz A, et al. J Vis Exp. 2017 Jun 6;(124):55745. doi: 10.3791/55745. J Vis Exp. 2017. PMID: 28654080 Free PMC article.
The synchronization protocol by hydroxyurea (HU) treatment leads to cellular arrest in late G1/early S phase, and release from HU-mediated arrest provides a cellular population uniformly progressing through S and G2/M. The synchronization protocol by thymidine and nocodazo …
The synchronization protocol by hydroxyurea (HU) treatment leads to cellular arrest in late G1/early S phase, and release from HU-mediated a …