A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging.
Farrants H, Shuai Y, Lemon WC, Monroy Hernandez C, Zhang D, Yang S, Patel R, Qiao G, Frei MS, Plutkis SE, Grimm JB, Hanson TL, Tomaska F, Turner GC, Stringer C, Keller PJ, Beyene AG, Chen Y, Liang Y, Lavis LD, Schreiter ER.
Farrants H, et al.
Nat Methods. 2024 Oct;21(10):1916-1925. doi: 10.1038/s41592-024-02411-6. Epub 2024 Sep 20.
Nat Methods. 2024.
PMID: 39304767
Free PMC article.
We use WHaloCaMP1a to image Ca(2+) responses in vivo in flies and mice, to perform three-color multiplexed functional imaging of hundreds of neurons and astrocytes in zebrafish larvae and to quantify Ca(2+) concentration using fluorescence lifetime imaging microscopy (FLIM …
We use WHaloCaMP1a to image Ca(2+) responses in vivo in flies and mice, to perform three-color multiplexed functional imaging of hundreds of …