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Electrostatic interactions at the five-fold axis alter heparin-binding phenotype and drive enterovirus A71 virulence in mice.
Tee HK, Tan CW, Yogarajah T, Lee MHP, Chai HJ, Hanapi NA, Yusof SR, Ong KC, Lee VS, Sam IC, Chan YF. Tee HK, et al. PLoS Pathog. 2019 Nov 15;15(11):e1007863. doi: 10.1371/journal.ppat.1007863. eCollection 2019 Nov. PLoS Pathog. 2019. PMID: 31730673 Free PMC article.
We constructed six viruses with varying residues at VP1-98, VP1-145 (which are both heparin-binding determinants) and VP2-149 (based on the wild type 149K/98E/145Q, termed KEQ) to generate KKQ, KKE, KEE, IEE and IEQ variants. We demonstrated that the weak heparin-binder IE …
We constructed six viruses with varying residues at VP1-98, VP1-145 (which are both heparin-binding determinants) and VP2-149 (based on the …