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Haloarcula hispanica CRISPR authenticates PAM of a target sequence to prime discriminative adaptation.
Li M, Wang R, Xiang H. Li M, et al. Nucleic Acids Res. 2014 Jun;42(11):7226-35. doi: 10.1093/nar/gku389. Epub 2014 May 6. Nucleic Acids Res. 2014. PMID: 24803673 Free PMC article.
The adaptation mechanism avoiding acquiring 'self' DNA fragments is poorly understood. In Haloarcula hispanica, we previously showed that CRISPR adaptation requires being primed by a pre-existing spacer partially matching the invader DNA. ...
The adaptation mechanism avoiding acquiring 'self' DNA fragments is poorly understood. In Haloarcula hispanica, we previously …
AepG is a glucuronosyltransferase involved in acidic exopolysaccharide synthesis and contributes to environmental adaptation of Haloarcula hispanica.
Pei C, Lu H, Ma J, Eichler J, Guan Z, Gao L, Liu L, Zhou H, Yang J, Jin C. Pei C, et al. J Biol Chem. 2023 Feb;299(2):102911. doi: 10.1016/j.jbc.2023.102911. Epub 2023 Jan 13. J Biol Chem. 2023. PMID: 36642187 Free PMC article.
The attachment of a sugar to a hydrophobic lipid carrier is the first step in the biosynthesis of many glycoconjugates. In the halophilic archaeon Haloarcula hispanica, HAH_1206, renamed AepG, is a predicted glycosyltransferase belonging to the CAZy Group 2 family t …
The attachment of a sugar to a hydrophobic lipid carrier is the first step in the biosynthesis of many glycoconjugates. In the halophilic ar …
Novel Insights Into the Sulfated Glucuronic Acid-Based Anti-SARS-CoV-2 Mechanism of Exopolysaccharides From Halophilic Archaeon Haloarcula hispanica.
Xu Y, Li Y, You X, Pei C, Wang Z, Jiao S, Zhao X, Lin X, Lü Y, Jin C, Gao GF, Li J, Wang Q, Du Y. Xu Y, et al. Front Chem. 2022 Apr 27;10:871509. doi: 10.3389/fchem.2022.871509. eCollection 2022. Front Chem. 2022. PMID: 35572116 Free PMC article.
In this study, it was found exopolysaccharides (EPSs) from halophilic archaeon Haloarcula hispanica ATCC33960 can bind to the spike protein of SARS-CoV-2 with the binding constant K(D) of 2.23 nM, block the binding of spike protein to Vero E6 and bronchial epithelia …
In this study, it was found exopolysaccharides (EPSs) from halophilic archaeon Haloarcula hispanica ATCC33960 can bind to the …
Genome Sequence of Hardyhisp2, a Gammapleolipovirus Infecting Haloarcula hispanica.
Dyall-Smith M, Pfeiffer F, Chiang PW, Tang SL. Dyall-Smith M, et al. Microbiol Resour Announc. 2021 May 13;10(19):e00226-21. doi: 10.1128/MRA.00226-21. Microbiol Resour Announc. 2021. PMID: 33986080 Free PMC article.
Hardyhisp2 virus infects the halophilic archaeon Haloarcula hispanica DSM 4426(T) and is closely related to His2 (Pleolipoviridae family). ...
Hardyhisp2 virus infects the halophilic archaeon Haloarcula hispanica DSM 4426(T) and is closely related to His2 (Pleolipoviri …
Insights into gene regulation of the halovirus His2 infecting Haloarcula hispanica.
Lee STM, Ding JY, Chiang PW, Dyall-Smith M, Tang SL. Lee STM, et al. Microbiologyopen. 2020 May;9(5):e1016. doi: 10.1002/mbo3.1016. Epub 2020 Mar 25. Microbiologyopen. 2020. PMID: 32212320 Free PMC article.
Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. ...
Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microar …
An Acidic Exopolysaccharide from Haloarcula hispanica ATCC33960 and Two Genes Responsible for Its Synthesis.
Lü Y, Lu H, Wang S, Han J, Xiang H, Jin C. Lü Y, et al. Archaea. 2017 May 28;2017:5842958. doi: 10.1155/2017/5842958. eCollection 2017. Archaea. 2017. PMID: 28634434 Free PMC article.
A 1.1 10(6) Da acidic exopolysaccharide (EPS) was purified from an extremely halophilic archaeon Haloarcula hispanica ATCC33960 with a production of 30 mg L(-1) when grown in AS-168 medium, which mainly composed of mannose and galactose with a small amount of glucos …
A 1.1 10(6) Da acidic exopolysaccharide (EPS) was purified from an extremely halophilic archaeon Haloarcula hispanica ATCC3396 …
Reprogramming the endogenous type I CRISPR-Cas system for simultaneous gene regulation and editing in Haloarcula hispanica.
Du K, Gong L, Li M, Yu H, Xiang H. Du K, et al. mLife. 2022 Mar 17;1(1):40-50. doi: 10.1002/mlf2.12010. eCollection 2022 Mar. mLife. 2022. PMID: 38818324 Free PMC article.
Here, we conducted a comprehensive study of the impact of redesigned crRNAs with different spacer lengths on gene regulation with the native type I-B CRISPR system in Haloarcula hispanica. When the spacer targeting the chromosomal gene was shortened from 36 to 28 bp …
Here, we conducted a comprehensive study of the impact of redesigned crRNAs with different spacer lengths on gene regulation with the native …
Vesicle-like virion of Haloarcula hispanica pleomorphic virus 3 preserves high infectivity in saturated salt.
Demina TA, Atanasova NS, Pietilä MK, Oksanen HM, Bamford DH. Demina TA, et al. Virology. 2016 Dec;499:40-51. doi: 10.1016/j.virol.2016.09.002. Epub 2016 Sep 12. Virology. 2016. PMID: 27632564 Free article.
Here we report a newly isolated archaeal halovirus, Haloarcula hispanica pleomorphic virus 3 (HHPV3). Its reproduction significantly retards host growth and decreases cell viability without causing lysis. ...
Here we report a newly isolated archaeal halovirus, Haloarcula hispanica pleomorphic virus 3 (HHPV3). Its reproduction signifi …
Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
Lu H, Pei C, Zhou H, Lü Y, He Y, Li Y, Han J, Xiang H, Eichler J, Jin C. Lu H, et al. Mol Microbiol. 2020 Nov;114(5):762-774. doi: 10.1111/mmi.14577. Epub 2020 Aug 10. Mol Microbiol. 2020. PMID: 32706435 Free article.
To explore commonalities and variations of these two versions of glycosylation, we used Haloarcula hispanica as a model. Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-alpha-(1, 2)-[sulfoquinovosamine-beta …
To explore commonalities and variations of these two versions of glycosylation, we used Haloarcula hispanica as a model. Our p …
Adaptation of the Haloarcula hispanica CRISPR-Cas system to a purified virus strictly requires a priming process.
Li M, Wang R, Zhao D, Xiang H. Li M, et al. Nucleic Acids Res. 2014 Feb;42(4):2483-92. doi: 10.1093/nar/gkt1154. Epub 2013 Nov 21. Nucleic Acids Res. 2014. PMID: 24265226 Free PMC article.
Infected by a newly isolated virus HHPV-2, Haloarcula hispanica type I-B CRISPR system acquired spacers discriminatively from viral sequences. ...
Infected by a newly isolated virus HHPV-2, Haloarcula hispanica type I-B CRISPR system acquired spacers discriminatively from …
83 results