Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells.
Rohani L, Borys BS, Razian G, Naghsh P, Liu S, Johnson AA, Machiraju P, Holland H, Lewis IA, Groves RA, Toms D, Gordon PMK, Li JW, So T, Dang T, Kallos MS, Rancourt DE.
Rohani L, et al.
Commun Biol. 2020 Sep 7;3(1):492. doi: 10.1038/s42003-020-01218-3.
Commun Biol. 2020.
PMID: 32895477
Free PMC article.
Because of differences in bioreactor expansion efficiency between mouse (m) and human (h) PSCs, we investigated if conversion of hPSCs, from the conventional "primed" pluripotent state towards the "naive" state prevalent in mPSCs, could be used to enhance hPSC production. …
Because of differences in bioreactor expansion efficiency between mouse (m) and human (h) PSCs, we investigated if conversion of hPSC …