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Purification and characterization of a hyperthermostable and high maltogenic alpha-amylase of an extreme thermophile Geobacillus thermoleovorans.
Uma Maheswar Rao JL, Satyanarayana T. Uma Maheswar Rao JL, et al. Appl Biochem Biotechnol. 2007 Aug;142(2):179-93. doi: 10.1007/s12010-007-0017-4. Appl Biochem Biotechnol. 2007. PMID: 18025579
Among different additives and detergents, polyethylene glycol 8000 and Tween 20, 40, and 80 stabilized the enzyme activity, whereas Triton X-100, glycerol, glycine, dextrin, and sodium dodecyl sulfate inhibited to a varied extent. alpha-Amylase exhibited activity on several starc …
Among different additives and detergents, polyethylene glycol 8000 and Tween 20, 40, and 80 stabilized the enzyme activity, whereas Triton X …
Biophysical and biochemical characterization of a hyperthermostable and Ca2+ -independent alpha-Amylase of an extreme thermophile Geobacillus thermoleovorans.
Uma Maheswar Rao JL, Satyanarayana T. Uma Maheswar Rao JL, et al. Appl Biochem Biotechnol. 2008 Aug;150(2):205-19. doi: 10.1007/s12010-008-8171-x. Epub 2008 Apr 29. Appl Biochem Biotechnol. 2008. PMID: 18443744
The addition of urea, at high concentration (8 M), appeared to expose the buried Trp residues of the native alpha-amylase gt to the aqueous environment and thus showed low fluorophore. ...
The addition of urea, at high concentration (8 M), appeared to expose the buried Trp residues of the native alpha-amylase gt to the a …