Native mass spectrometry identifies an alternative DNA-binding pathway for BirA from Staphylococcus aureus.
Satiaputra J, Sternicki LM, Hayes AJ, Pukala TL, Booker GW, Shearwin KE, Polyak SW.
Satiaputra J, et al.
Sci Rep. 2019 Feb 26;9(1):2767. doi: 10.1038/s41598-019-39398-6.
Sci Rep. 2019.
PMID: 30808984
Free PMC article.
Here, we employ a combination of native mass spectrometry, in vivo gene expression assays, site-directed mutagenesis and electrophoretic mobility shift assays to elucidate the DNA binding pathway for S. aureus BirA (SaBirA). We identify a mechanism that differs from that o …
Here, we employ a combination of native mass spectrometry, in vivo gene expression assays, site-directed mutagenesis and electrophoretic mob …