Voltage Rollercoaster Filtering of Low-Mass Contaminants During Native Protein Analysis.
McGee JP, Melani RD, Goodwin M, McAlister G, Huguet R, Senko MW, Compton PD, Kelleher NL.
McGee JP, et al.
J Am Soc Mass Spectrom. 2020 Mar 4;31(3):763-767. doi: 10.1021/jasms.9b00037. Epub 2020 Feb 24.
J Am Soc Mass Spectrom. 2020.
PMID: 32126774
Free PMC article.
Using this method, we show complete filtration of 2 mM polyethylene glycol (PEG-1000) during native MS of SILu mAb antibody present at a 200 lower concentration. We also show the generality of the process by rescuing 10 muM tetrameric pyruvate kinase from 2 mM PEG-1000, as …
Using this method, we show complete filtration of 2 mM polyethylene glycol (PEG-1000) during native MS of SILu mAb antibody present a …