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Peri-nuclear antibodies correlate with survival in Greek primary biliary cirrhosis patients.
Sfakianaki O, Koulentaki M, Tzardi M, Tsangaridou E, Theodoropoulos PA, Castanas E, Kouroumalis EA. Sfakianaki O, et al. World J Gastroenterol. 2010 Oct 21;16(39):4938-43. doi: 10.3748/wjg.v16.i39.4938. World J Gastroenterol. 2010. PMID: 20954280 Free PMC article.
ANEA were detected with indirect immunofluorescence on 1% formaldehyde fixed Hep2 cells, and anti-gp210 antibodies were detected using an enzyme linked immunosorbent assay. Findings were correlated with clinical data, histology, and survival. ...
ANEA were detected with indirect immunofluorescence on 1% formaldehyde fixed Hep2 cells, and anti-gp210 antibodies were detected using an …
Differential detection of nuclear envelope autoantibodies in primary biliary cirrhosis using routine and alternative methods.
Tsangaridou E, Polioudaki H, Sfakianaki R, Samiotaki M, Tzardi M, Koulentaki M, Panayotou G, Kouroumalis E, Castanas E, Theodoropoulos PA. Tsangaridou E, et al. BMC Gastroenterol. 2010 Mar 8;10:28. doi: 10.1186/1471-230X-10-28. BMC Gastroenterol. 2010. PMID: 20205958 Free PMC article.
Anti-gp210 antibodies were identified in 22.3% and 33% of sera using ELISA for the C-terminal of gp210 or both ELISA and immunoprecipitation, respectively. ...CONCLUSIONS: The prevalence of ANEA or anti-gp210 antibodies is under-estimated in PBC sera which are analy …
Anti-gp210 antibodies were identified in 22.3% and 33% of sera using ELISA for the C-terminal of gp210 or both ELISA and immun …
Matrix metalloproteinases and their inhibitors in acute viral hepatitis.
Koulentaki M, Valatas V, Xidakis K, Kouroumalis A, Petinaki E, Castanas E, Kouroumalis E. Koulentaki M, et al. J Viral Hepat. 2002 May;9(3):189-93. doi: 10.1046/j.1365-2893.2002.00351.x. J Viral Hepat. 2002. PMID: 12010506
Serum levels of TGF-beta, IL-10, MMP-1, MMP-3, MMP-2, MMP-9, TIMP-1 and TIMP-2 were assessed by ELISA. MMP-2 and MMP-9 were also measured by a zymogram protease assay. alpha2 macroglobulin (AMG) was measured by nephelometry. ...
Serum levels of TGF-beta, IL-10, MMP-1, MMP-3, MMP-2, MMP-9, TIMP-1 and TIMP-2 were assessed by ELISA. MMP-2 and MMP-9 were also meas …
Androgen Triggers the Pro-Migratory CXCL12/CXCR4 Axis in AR-Positive Breast Cancer Cell Lines: Underlying Mechanism and Possible Implications for the Use of Aromatase Inhibitors in Breast Cancer.
Azariadis K, Kiagiadaki F, Pelekanou V, Bempi V, Alexakis K, Kampa M, Tsapis A, Castanas E, Notas G. Azariadis K, et al. Cell Physiol Biochem. 2017;44(1):66-84. doi: 10.1159/000484584. Epub 2017 Nov 3. Cell Physiol Biochem. 2017. PMID: 29131020 Free article.
METHODS: We analyzed the expression of CXCL12 and its receptors with qPCR and ELISA and the role of Nuclear Receptor Coactivator 1 (NCOA1) in this effect. ...
METHODS: We analyzed the expression of CXCL12 and its receptors with qPCR and ELISA and the role of Nuclear Receptor Coactivator 1 (N …
Comparison of a multiplex, bead-based fluorescent assay and immunofluorescence methods for the detection of ANA and ANCA autoantibodies in human serum.
Nifli AP, Notas G, Mamoulaki M, Niniraki M, Ampartzaki V, Theodoropoulos PA, Kopnitsky MJ, Castanas E. Nifli AP, et al. J Immunol Methods. 2006 Apr 20;311(1-2):189-97. doi: 10.1016/j.jim.2006.02.004. Epub 2006 Mar 3. J Immunol Methods. 2006. PMID: 16554066
Recently a commercially available microsphere-based fluorescent assay has been introduced for the detection of ANA and ANCA. The aim of this study was to compare this technology with routinely used IIF and ELISA procedures, in patients with a suggested autoimmune di …
Recently a commercially available microsphere-based fluorescent assay has been introduced for the detection of ANA and ANCA. The aim …
Anti-Saccharomyces cerevisiae mannan antibodies and antineutrophil cytoplasmic autoantibodies in Greek patients with inflammatory bowel disease.
Koutroubakis IE, Petinaki E, Mouzas IA, Vlachonikolis IG, Anagnostopoulou E, Castanas E, Maniatis AN, Kouroumalis EA. Koutroubakis IE, et al. Am J Gastroenterol. 2001 Feb;96(2):449-54. doi: 10.1111/j.1572-0241.2001.03524.x. Am J Gastroenterol. 2001. PMID: 11232689
Determination of pANCA was performed by a standard indirect immunofluorescence technique on ethanol-fixed granulocytes and ASCA by an ELISA assay. RESULTS: In patients with UC, sensitivity, specificity, positive predictive value, and negative predictive value of the …
Determination of pANCA was performed by a standard indirect immunofluorescence technique on ethanol-fixed granulocytes and ASCA by an ELI