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High-throughput, high-force probing of DNA-protein interactions with magnetic tweezers.
Berghuis BA, Köber M, van Laar T, Dekker NH. Berghuis BA, et al. Among authors: van laar t. Methods. 2016 Aug 1;105:90-8. doi: 10.1016/j.ymeth.2016.03.025. Epub 2016 Mar 30. Methods. 2016. PMID: 27038745 Free article.
Firstly, we discuss a DNA hairpin assay where force-induced strand separation triggers a tight interaction between DNA-binding protein Tus and its binding site Ter, where forces up to 90pN for hundreds of seconds were required to dissociate Tus from Ter. ...
Firstly, we discuss a DNA hairpin assay where force-induced strand separation triggers a tight interaction between DNA-binding protein Tus a …
Strand separation establishes a sustained lock at the Tus-Ter replication fork barrier.
Berghuis BA, Dulin D, Xu ZQ, van Laar T, Cross B, Janissen R, Jergic S, Dixon NE, Depken M, Dekker NH. Berghuis BA, et al. Among authors: van laar t. Nat Chem Biol. 2015 Aug;11(8):579-85. doi: 10.1038/nchembio.1857. Epub 2015 Jul 6. Nat Chem Biol. 2015. PMID: 26147356
In Escherichia coli, replisome progression beyond the termination site is prevented by Tus proteins bound to asymmetric Ter sites. Structural evidence indicates that strand separation on the blocking (nonpermissive) side of Tus-Ter triggers roadblock formation, but …
In Escherichia coli, replisome progression beyond the termination site is prevented by Tus proteins bound to asymmetric Ter sites. St …
Impact of Metabolic Bariatric Surgery on Weight Loss and Glycemic Control in Adults With Type 1 Diabetes: A Multicenter Retrospective Cohort Study.
van der Meer R, Pazmino S, Steenackers N, van Laar A, Sluis M, Tolenaars L, Rosen J, Hurtado Del Pozo C, le Roux CW, Al Kandari J, Irshad M, Lannoo M, Deleus E, Dillemans B, Van Nieuwenhove Y, van Bon A, Hazebroek E, Al Ozairi E, Hoogma R, Mathieu C, Liem R, Van der Schueren B. van der Meer R, et al. Among authors: van laar a. Diabetes Care. 2026 Apr 1;49(4):658-663. doi: 10.2337/dc25-2295. Diabetes Care. 2026. PMID: 41706050 Free PMC article.