A molecularly imprinted polymerized high internal phase emulsion adsorbent for sensitive chemiluminescence determination of clopidogrel

A molecularly imprinted polymerized high internal phase emulsion (MIP-polyHIPE) adsorbent was used for selective separating and preconcentrating the anti-plaque drug, clopidogrel. For the first time in this study, chemiluminescence (CL) methods were evaluated for the determination of clopidogrel. The synthesis of adsorbents by the emulsion templating method showed that the sensitivity of the method can be increased up to 42 times. The determination of clopidogrel was evaluated by Ru(phen)₃²⁺-Cerium (IV), KMnO₄-H₂SO₄, KMnO₄-H₂SO₄-Na₂SO₃, and luminol-H₂O₂ CL systems. According to the results, only the Ru(phen)₃²⁺-Cerium (IV) CL system showed a reasonable sensitivity for clopidogrel. Using MIP-polyHIPE adsorbent, the linear range, the limit of detection, and relative standard deviation for clopidogrel in this system were respectively 1.0 × 10^-9-8.0 × 10^-8 mol L^-1, 3.0 × 10^-10 mol L^-1, and 6.3% (n = 4, 1.0 × 10^-8). The proposed method was employed for determining clopidogrel in pharmaceuticals and blood serum samples. The results showed the good sensitivity and accuracy of the proposed method.