Rational design toward developing a more efficient laccase: Catalytic efficiency and selectivity.
Khodakarami A, Goodarzi N, Hoseinzadehdehkordi M, Amani F, Khodaverdian S, Khajeh K, Ghazi F, Ranjbar B, Amanlou M, Dabirmanesh B.
Khodakarami A, et al.
Int J Biol Macromol. 2018 Jun;112:775-779. doi: 10.1016/j.ijbiomac.2018.02.019. Epub 2018 Feb 6.
Int J Biol Macromol. 2018.
PMID: 29425870
Based on the ABTS-bound crystal structure of CotA from B. subtilis and alignment with closely related enzymes, T415 and T418 at the vicinity of the type 1 copper site were chosen and several mutants (T415I, T418I, T415G, T415G/T418I) were made. ...
Based on the ABTS-bound crystal structure of CotA from B. subtilis and alignment with closely related enzymes, T415 and T418 at the v …