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Downregulation of circRNA DMNT3B contributes to diabetic retinal vascular dysfunction through targeting miR-20b-5p and BAMBI.
Zhu K, Hu X, Chen H, Li F, Yin N, Liu AL, Shan K, Qin YW, Huang X, Chang Q, Xu GZ, Wang Z. Zhu K, et al. EBioMedicine. 2019 Nov;49:341-353. doi: 10.1016/j.ebiom.2019.10.004. Epub 2019 Oct 19. EBioMedicine. 2019. PMID: 31636010 Free PMC article.
METHODS: qRT-PCR was performed to detect expression of circDNMT3B, miR-20b-5p, and BAMBI in retinal microvascular endothelial cells under diabetic conditions. ...Changes in expression of circDNMT3B and miR-20b-5p were confirmed in the proliferative fibrovascular mem …
METHODS: qRT-PCR was performed to detect expression of circDNMT3B, miR-20b-5p, and BAMBI in retinal microvascular endothelial cells u …
Decreased expression of microRNA-17 and microRNA-20b promotes breast cancer resistance to taxol therapy by upregulation of NCOA3.
Ao X, Nie P, Wu B, Xu W, Zhang T, Wang S, Chang H, Zou Z. Ao X, et al. Cell Death Dis. 2016 Nov 10;7(11):e2463. doi: 10.1038/cddis.2016.367. Cell Death Dis. 2016. PMID: 27831559 Free PMC article.
By bioinformatics prediction in combination with the data of previous report, miR-17 and miR-20b were selected as the potential miRNAs targeting NCOA3. By real-time PCR analysis, we found that miR-17 and miR-20b were significantly reduced in taxol-resistant breast c …
By bioinformatics prediction in combination with the data of previous report, miR-17 and miR-20b were selected as the potential miRNA …
Identification of a novel microRNA profile including miR-106b, miR-17, miR-20b, miR-18a and miR-93 in the metastasis of nasopharyngeal carcinoma.
Zhou W, Chang A, Zhao H, Ye H, Li D, Zhuo X. Zhou W, et al. Cancer Biomark. 2020;27(4):533-539. doi: 10.3233/CBM-190601. Cancer Biomark. 2020. PMID: 32083569
Among them, eleven hub genes were identified, such as PTEN, KAT2B, CCND1, STAT3, and MAP3K5. Moreover, a five-miRNA profile (miR-106b, miR-17, miR-20b, miR-18a and miR-93) was identified and their expression levels were tested to be up-regulated in high-metastatic NPC cell …
Among them, eleven hub genes were identified, such as PTEN, KAT2B, CCND1, STAT3, and MAP3K5. Moreover, a five-miRNA profile (miR-106b, miR-1 …
A novel platform for discovery of differentially expressed microRNAs in patients with repeated implantation failure.
Chen CH, Lu F, Yang WJ, Yang PE, Chen WM, Kang ST, Huang YS, Kao YC, Feng CT, Chang PC, Wang T, Hsieh CA, Lin YC, Jen Huang JY, Wang LH. Chen CH, et al. Fertil Steril. 2021 Jul;116(1):181-188. doi: 10.1016/j.fertnstert.2021.01.055. Epub 2021 Apr 3. Fertil Steril. 2021. PMID: 33823989 Free article.
RESULT(S): We designed a reproductive disease-related PanelChip to access endometrium miRNA profiles in patients undergoing IVF. Three major miRNA signatures, including hsa-miR-20b-5p, hsa-miR-155-5p, and hsa-miR-718, were identified using infinite combination signature se …
RESULT(S): We designed a reproductive disease-related PanelChip to access endometrium miRNA profiles in patients undergoing IVF. Three major …
cDNA sequence analysis and expression of cardiotoxins from Taiwan Cobra.
Chang LS, Wu PF, Lin J. Chang LS, et al. Biochem Biophys Res Commun. 1996 Feb 6;219(1):116-21. doi: 10.1006/bbrc.1996.0191. Biochem Biophys Res Commun. 1996. PMID: 8619792
A high degree of sequence homology was observed with the three cardiotoxins. The cardiotoxins were subcloned in the expression vector pET 20b(+) and transformed in BL 21(DE3) E. coli strain. The expressed protein was isolated from the inclusion bodies of E. coli and purifi …
A high degree of sequence homology was observed with the three cardiotoxins. The cardiotoxins were subcloned in the expression vector pET …
Profiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture.
Wu SC, Yang JC, Rau CS, Chen YC, Lu TH, Lin MW, Tzeng SL, Wu YC, Wu CJ, Hsieh CH. Wu SC, et al. PLoS One. 2013 Oct 30;8(10):e77936. doi: 10.1371/journal.pone.0077936. eCollection 2013. PLoS One. 2013. PMID: 24205035 Free PMC article.
Microarray analysis revealed upregulation of 10 miRNA targets (miR-16, miR-17, miR-20a, miR-20b, miR-26a, miR-26b, miR-106a, miR-106b, miR-195, and miR-451). ...Immunoprecipitation with the Argonaute 2 ribonucleoprotein complex revealed significantly increased expression o …
Microarray analysis revealed upregulation of 10 miRNA targets (miR-16, miR-17, miR-20a, miR-20b, miR-26a, miR-26b, miR-106a, miR-106b …
MicroRNA-34a modulates genes involved in cellular motility and oxidative phosphorylation in neural precursors derived from human umbilical cord mesenchymal stem cells.
Chang SJ, Weng SL, Hsieh JY, Wang TY, Chang MD, Wang HW. Chang SJ, et al. BMC Med Genomics. 2011 Sep 19;4:65. doi: 10.1186/1755-8794-4-65. BMC Med Genomics. 2011. PMID: 21923954 Free PMC article.
RESULTS: Five miRNAs were enriched in WJ-MSCs, including miR-345, miR-106a, miR-17-5p, miR-20a and miR-20b. Another 11 miRNAs (miR-206, miR-34a, miR-374, miR-424, miR-100, miR-101, miR-323, miR-368, miR-137, miR-138 and miR-377) were abundantly expressed in transdifferenti …
RESULTS: Five miRNAs were enriched in WJ-MSCs, including miR-345, miR-106a, miR-17-5p, miR-20a and miR-20b. Another 11 miRNAs (miR-20 …
Copper/zinc-superoxide dismutase from Epinephelus malabaricus cDNA and enzyme property.
Ken CF, Cheng YF, Chang CF, Lin CT. Ken CF, et al. J Agric Food Chem. 2003 Sep 10;51(19):5688-94. doi: 10.1021/jf030162p. J Agric Food Chem. 2003. PMID: 12952420
To further characterize the E. malabaricus Cu/Zn-SOD, the coding region was subcloned into an expression vector, pET-20b(+) and transformed into Escherichia coli BL21(DE3)pLysS. The expression of the Cu/Zn-SOD was confirmed by enzyme activity stained on a native gel and pu …
To further characterize the E. malabaricus Cu/Zn-SOD, the coding region was subcloned into an expression vector, pET-20b(+) and trans …
Expression pattern of metallothionein, MTF-1 nuclear translocation, and its dna-binding activity in zebrafish (Danio rerio) induced by zinc and cadmium.
Chen WY, John JA, Lin CH, Chang CY. Chen WY, et al. Environ Toxicol Chem. 2007 Jan;26(1):110-7. doi: 10.1897/06-153r.1. Environ Toxicol Chem. 2007. PMID: 17269467
The MTF-1 protein was produced recombinantly by inserting zebrafish mtf-1 cDNA (1.8 kb) into pET-20b(+) expression vector and expressing in Escherichia coli BL21 (DE3) pLysS host strain competent cell on induction with isopropyl-beta-D-thiogalactopyranoside. ...
The MTF-1 protein was produced recombinantly by inserting zebrafish mtf-1 cDNA (1.8 kb) into pET-20b(+) expression vector and express …
13 results