Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer.
Couturier A, Virolle C, Goldlust K, Berne-Dedieu A, Reuter A, Nolivos S, Yamaichi Y, Bigot S, Lesterlin C.
Couturier A, et al.
Nat Commun. 2023 Jan 18;14(1):294. doi: 10.1038/s41467-023-35978-3.
Nat Commun. 2023.
PMID: 36653393
Free PMC article.
Here, we use live-cell microscopy to visualise the intracellular dynamics of conjugative transfer of F-plasmid in E. coli, in real time. We show that the transfer of plasmid in single-stranded form (ssDNA) and its subsequent conversion into double-stranded DNA (dsDNA) are …
Here, we use live-cell microscopy to visualise the intracellular dynamics of conjugative transfer of F-plasmid in E. coli, in real ti …