Luminescent Human iPSC-Derived Neurospheroids Enable Modeling of Neurotoxicity After Oxygen-glucose Deprivation.
Van Breedam E, Nijak A, Buyle-Huybrecht T, Di Stefano J, Boeren M, Govaerts J, Quarta A, Swartenbroekx T, Jacobs EZ, Menten B, Gijsbers R, Delputte P, Alaerts M, Hassannia B, Loeys B, Berneman Z, Timmermans JP, Jorens PG, Vanden Berghe T, Fransen E, Wouters A, De Vos WH, Ponsaerts P.
Van Breedam E, et al.
Neurotherapeutics. 2022 Mar;19(2):550-569. doi: 10.1007/s13311-022-01212-z. Epub 2022 Mar 14.
Neurotherapeutics. 2022.
PMID: 35289376
Free PMC article.
We subjected 1- and 4-week-old neurospheroids, generated from iPSC-derived neural stem cells, to 6 h of oxygen-glucose deprivation (OGD) and measured neurospheroid luminescence. For both, we detected a decrease in luminescent signal due to ensuing neurotoxicity, as confirm …
We subjected 1- and 4-week-old neurospheroids, generated from iPSC-derived neural stem cells, to 6 h of oxygen-glucose deprivation (O …