An engineered cellobiohydrolase I for sustainable degradation of lignocellulosic biomass.
Pramanik S, Semenova MV, M Rozhkova A, Zorov IN, Korotkova O, Sinitsyn AP, Davari MD.
Pramanik S, et al.
Biotechnol Bioeng. 2021 Oct;118(10):4014-4027. doi: 10.1002/bit.27877. Epub 2021 Jul 12.
Biotechnol Bioeng. 2021.
PMID: 34196389
Engineered triple variant CBH-I R1 (A65R-G415R-S181F) showed 2.48-fold higher thermostability in terms of relative activity at 65C after 1 h of incubation when compared with CBH-I wild type. CBH-I R1 exhibited 1.87-fold, 1.36-fold, and 1.57-fold higher specific activities …
Engineered triple variant CBH-I R1 (A65R-G415R-S181F) showed 2.48-fold higher thermostability in terms of relative activity at 65C after 1 …