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Ninety-six-hour starved peripheral blood mononuclear cell supernatant inhibited LA7 breast cancer stem cells induced tumor via reduction in angiogenesis and alternations in Gch1 and Spr expressions.
Mehri M, Gheitasi R, Pourbagher R, Ranaee M, Nayeri K, Rahimi SM, Khorasani HR, Hossein-Nattaj H, Sabour D, Akhavan-Niaki H, Fattahi S, Kalali B, Mostafazadeh A. Mehri M, et al. Front Immunol. 2023 Feb 23;13:1025933. doi: 10.3389/fimmu.2022.1025933. eCollection 2022. Front Immunol. 2023. PMID: 36908807 Free PMC article.
METHOD: Ninety-six-hour starved PBMCs supernatant (96 h-SPS) was collected after culturing human PBMCs for 96 h under serum starvation condition. ...Last, proteomics analysis revealed candidate effectors' components of 96 h-SPS solution. CONCLUSION: 96 h …
METHOD: Ninety-six-hour starved PBMCs supernatant (96 h-SPS) was collected after culturing human PBMCs for 96 h under serum st …
The effect of eliminating sintering during the synthesis of 3D scaffolds using the gel-casting method on their biological characteristics:in vivoandin vitroevaluations.
Seyedmajidi S, Pourbagher R, Seyedmajidi M. Seyedmajidi S, et al. Biomed Mater. 2023 Aug 3;18(5). doi: 10.1088/1748-605X/acea0f. Biomed Mater. 2023. PMID: 37487494
To assessin vitrocytotoxicity, the supernatant culture medium was extracted from 100 mg ml(-1)foam suspension in complete culture medium after 72 h incubation and diluted into various concentrations. SaOs-II cells were incubated with extracts of each scaffold at different …
To assessin vitrocytotoxicity, the supernatant culture medium was extracted from 100 mg ml(-1)foam suspension in complete culture medium aft …
Targeting LA7 breast cancer stem cells of rat through repressing the genes of stemness-related transcription factors using three different biological fluids.
Pourbagher R, Akhavan-Niaki H, Jorsaraei SGA, Fattahi S, Sabour D, Zabihi E, Abedian Z, Ghasemi M, Golpour M, Mostafazadeh A. Pourbagher R, et al. Gene. 2020 Apr 15;734:144381. doi: 10.1016/j.gene.2020.144381. Epub 2020 Jan 21. Gene. 2020. PMID: 31978510
The aim of this study was to evaluate the Oct4 , Sox2, Nanog, and C-myc expression in rat breast cancer stem cells (LA7) which treated with human ovarian follicular fluid (FF), replicative senescent fibroblast culture supernatant (P14), and 16 h serum starved fibroblast su …
The aim of this study was to evaluate the Oct4 , Sox2, Nanog, and C-myc expression in rat breast cancer stem cells (LA7) which treated with …
Anticancer properties of chitosan against osteosarcoma, breast cancer and cervical cancer cell lines.
Abedian Z, Moghadamnia AA, Zabihi E, Pourbagher R, Ghasemi M, Nouri HR, Tashakorian H, Jenabian N. Abedian Z, et al. Caspian J Intern Med. 2019 Fall;10(4):439-446. doi: 10.22088/cjim.10.4.439. Caspian J Intern Med. 2019. PMID: 31814943 Free PMC article.
The cells were treated by different concentration of chitosan and viability was determined by MTT assay after 24, 48 and 72 h .Also the mode of cell death-apoptosis vs necrosis ,was determined by Annexin V staining assay and analyzed by flow cytometry. ...
The cells were treated by different concentration of chitosan and viability was determined by MTT assay after 24, 48 and 72 h .Also t …
Central effects of camphor on GnRH and sexual hormones in male rat.
Shahabi S, Jorsaraei SG, Moghadamnia AA, Zabihi E, Aghajanpour SM, Mousavi Kani SN, Pourbagher R, Hosseini SA, Esmaili M, Yoonesi AA, Zarghami A, Alinezhad F. Shahabi S, et al. Int J Mol Cell Med. 2012 Fall;1(4):191-6. Int J Mol Cell Med. 2012. PMID: 24551777 Free PMC article.
Downregulation of Stemness Genes and Induction of Necrosis in Rat LA7 Cancer Stem Cells Induced Tumors Treated with Starved Fibroblasts Culture Supernatant.
Pourbagher R, Ghorbani H, Akhavan-Niaki H, Jorsaraei SGA, Fattahi S, Ghooran S, Abedian Z, Ghasemi M, Saeedi F, Jafari N, Kalali B, Mostafazadeh A. Pourbagher R, et al. Rep Biochem Mol Biol. 2021 Apr;10(1):105-118. doi: 10.52547/rbmb.10.1.105. Rep Biochem Mol Biol. 2021. PMID: 34277874 Free PMC article.
Then the tumors were removed and divided into the two parts, one part was used to quantify gene expression by stem-loop RT-qPCR assay and the other part was used for Hematoxylin & Eosin (H & E), Giemsa, and immunohistochemistry (IHC) staining. RESULTS: All induced …
Then the tumors were removed and divided into the two parts, one part was used to quantify gene expression by stem-loop RT-qPCR assay and th …
Ultrasonographic evaluation of gallbladder functions in patients with irritable bowel syndrome.
Güçlü M, Pourbagher A, Serin E, Kul K, Ozer B, Cosar A, Içer MO, Gür G, Boyacioglu S. Güçlü M, et al. J Gastroenterol Hepatol. 2006 Aug;21(8):1309-12. doi: 10.1111/j.1440-1746.2006.04136.x. J Gastroenterol Hepatol. 2006. PMID: 16872315
RESULTS: In IBS patients and healthy controls, fasting gallbladder volumes were 19.64 +/- 3.09 mL and 20.04 +/- 1.82 mL, and residual volumes after 3 h were 3.36 +/- 6.82 mL and 9.74 +/- 1.50 mL. Except for the residual volume after 3 h, all these results were not s …
RESULTS: In IBS patients and healthy controls, fasting gallbladder volumes were 19.64 +/- 3.09 mL and 20.04 +/- 1.82 mL, and residual volume …
The Effects of Pre-Treatment and Post-Treatment of Thymol against tert-Butyl Hydroperoxide (t-BHP) Cytotoxicity in MCF-7 Cell Line and Fibroblast Derived Foreskin.
Dashtaki A, Mahjoub S, Zabihi E, Pourbagher R. Dashtaki A, et al. Rep Biochem Mol Biol. 2020 Oct;9(3):338-347. doi: 10.29252/rbmb.9.3.338. Rep Biochem Mol Biol. 2020. PMID: 33649728 Free PMC article.
METHODS: In the pre-treatment, MCF-7 and fibroblast cells were treated with various Thymol concentrations and incubated for 24 h. Then, t-BHP was added to a final concentration of 50 muM, and the cells were incubated for one h. In the post-treatment, cells were incu …
METHODS: In the pre-treatment, MCF-7 and fibroblast cells were treated with various Thymol concentrations and incubated for 24 h. The …