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192 results

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Page 1
Autoregulation of SafA Assembly through Recruitment of a Protein Cross-Linking Enzyme.
Fernandes CG, Moran CP Jr, Henriques AO. Fernandes CG, et al. J Bacteriol. 2018 Jun 25;200(14):e00066-18. doi: 10.1128/JB.00066-18. Print 2018 Jul 15. J Bacteriol. 2018. PMID: 29712873 Free PMC article.
We show that SafA and C30 are distributed between the coat and cortex layers. The deletion of tgl increases the extractability of SafA, mainly from the cortex. ...The localization of Tgl-cyan fluorescent protein (Tgl-CFP) is strongly, but not exclusively, dependent …
We show that SafA and C30 are distributed between the coat and cortex layers. The deletion of tgl increases the extractability of …
Interaction between coat morphogenetic proteins SafA and SpoVID.
Costa T, Isidro AL, Moran CP Jr, Henriques AO. Costa T, et al. J Bacteriol. 2006 Nov;188(22):7731-41. doi: 10.1128/JB.00761-06. Epub 2006 Sep 1. J Bacteriol. 2006. PMID: 16950916 Free PMC article.
Here, we show that the interaction between SafA and SpoVID involves the PYYH motif (region B) but also a 13-amino-acid region (region A) just downstream of the N-terminal LysM domain of SafA. ...In contrast, lesions in region A impaired the interaction of SafA …
Here, we show that the interaction between SafA and SpoVID involves the PYYH motif (region B) but also a 13-amino-acid region (region …
Morphogenetic proteins SpoVID and SafA form a complex during assembly of the Bacillus subtilis spore coat.
Ozin AJ, Henriques AO, Yi H, Moran CP Jr. Ozin AJ, et al. J Bacteriol. 2000 Apr;182(7):1828-33. doi: 10.1128/JB.182.7.1828-1833.2000. J Bacteriol. 2000. PMID: 10714986 Free PMC article.
We have extended these studies with the finding that SafA and SpoVID were coimmunoprecipitated by anti-SafA or anti-SpoVID antiserum from whole-cell extracts 3 and 4 h after the onset of sporulation. ...SafA appears to have a modular design. The C-term …
We have extended these studies with the finding that SafA and SpoVID were coimmunoprecipitated by anti-SafA or anti-SpoVID ant …
Identification and characterization of the Bacillus subtilis spore germination protein GerY.
Ramírez-Guadiana FH, Brogan AP, Rudner DZ. Ramírez-Guadiana FH, et al. J Bacteriol. 2024 Dec 19;206(12):e0039924. doi: 10.1128/jb.00399-24. Epub 2024 Nov 12. J Bacteriol. 2024. PMID: 39530705 Free PMC article.
A YFP-GerY fusion localizes around the developing and mature spore in a manner that depends on CotE and SafA, indicating that it is a component of the spore coat. Coat proteins encoded by the gerP operon and gerT are also required for efficient germination, and we show tha …
A YFP-GerY fusion localizes around the developing and mature spore in a manner that depends on CotE and SafA, indicating that it is a …
SwsB and SafA Are Required for CwlJ-Dependent Spore Germination in Bacillus subtilis.
Amon JD, Yadav AK, Ramirez-Guadiana FH, Meeske AJ, Cava F, Rudner DZ. Amon JD, et al. J Bacteriol. 2020 Feb 25;202(6):e00668-19. doi: 10.1128/JB.00668-19. Print 2020 Feb 25. J Bacteriol. 2020. PMID: 31871031 Free PMC article.
Other than the previously characterized protein YpeB, no additional factors were found to be specifically required for SleB activity. In contrast, our screen identified SafA and YlxY (renamed SwsB) in addition to the known factors GerQ and CotE as proteins required for Cwl …
Other than the previously characterized protein YpeB, no additional factors were found to be specifically required for SleB activity. In con …
SpoVID guides SafA to the spore coat in Bacillus subtilis.
Ozin AJ, Samford CS, Henriques AO, Moran CP Jr. Ozin AJ, et al. J Bacteriol. 2001 May;183(10):3041-9. doi: 10.1128/JB.183.10.3041-3049.2001. J Bacteriol. 2001. PMID: 11325931 Free PMC article.
In this study we examined the interaction between SpoVID and another spore coat protein, SafA, in vivo using the yeast two-hybrid system and in vitro. We found evidence that SpoVID and SafA directly interact and that SafA interacts with itself. Immunofluoresc …
In this study we examined the interaction between SpoVID and another spore coat protein, SafA, in vivo using the yeast two-hybrid sys …
A conserved signal-peptidase antagonist modulates membrane homeostasis of actinobacterial sortase critical for surface morphogenesis.
Ramirez NA, Wu C, Chang C, Siegel SD, Das A, Ton-That H. Ramirez NA, et al. Proc Natl Acad Sci U S A. 2022 Jul 12;119(28):e2203114119. doi: 10.1073/pnas.2203114119. Epub 2022 Jul 5. Proc Natl Acad Sci U S A. 2022. PMID: 35787040 Free PMC article.
The pleiotropic defect of the safA mutant is rescued by ectopic expression of safA from not only A. oris, but also Corynebacterium diphtheriae or Corynebacterium matruchotii. ...Bacterial two-hybrid analysis demonstrates that SafA and SrtA directly interact. …
The pleiotropic defect of the safA mutant is rescued by ectopic expression of safA from not only A. oris, but also Corynebacte …
192 results