Synergists action of piperonyl butoxide and S,S,S-tributyl phosphorotrithioate on toxicity of carbamate insecticides against Blattella germanica

Alireza Sanei Dehkordi; Yaser Salim Abadi; Hasan Nasirian; Teymour Hazratian; Mohammad Amin Gorouhi; Saideh Yousefi; Azim Paksa

doi:10.1016/j.apjtm.2017.09.010

Synergists action of piperonyl butoxide and S,S,S-tributyl phosphorotrithioate on toxicity of carbamate insecticides against Blattella germanica

Asian Pac J Trop Med. 2017 Oct;10(10):981-986. doi: 10.1016/j.apjtm.2017.09.010. Epub 2017 Sep 18.

Authors

Alireza Sanei Dehkordi¹, Yaser Salim Abadi², Hasan Nasirian³, Teymour Hazratian⁴, Mohammad Amin Gorouhi⁵, Saideh Yousefi⁶, Azim Paksa⁷

Affiliations

¹ Department of Medical Entomology and Vector Control, Faculty of Health, Hormozgan University of Medical Sciences, Bandar Abbas, Iran; Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
² Department of Health Services and Health Promotion, School of Health, Rafsanjan University of Medical Sciences, Rafsanjan, Iran; Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
³ Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
⁴ Tabriz University of Medical Sciences, Tabriz, Iran.
⁵ Department of Medical Entomology and Vector Control, School of Health, Kerman University of Medical Sciences, Kerman, Iran.
⁶ Sirjan Faculty of Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran.
⁷ Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran. Electronic address: a.paksa@yahoo.com.

PMID: 29111194
DOI: 10.1016/j.apjtm.2017.09.010

Abstract

Objective: To determine the synergists action of piperonyl butoxide (PBO) and S,S,S-tributyl phosphorotrithioate (DEF) on toxicity of carbamate insecticides against Blattella germanica in Tehran city.

Methods: In the current study, German cockroach strains were collected from several hospitals and dormitories in Tehran. At the beginning, different concentrations of bendiocarb and carbaryl (insecticides belong to carbamate group) were determined by surface contact on a susceptible strain. Then, the level of susceptibility and type of resistance mechanisms in the collected strains from contaminated sites to the aforementioned insecticides were studied by using PBO and DEF synergists with different insecticide ratios to synergist (1:0, 1:1, 1:2, 1:3).

Results: The DEF synergist along with bendiocarb and carbaryl completely eliminated the resistance in all strains but PBO did not completely eliminate the resistance in the strains of Mofid, Alvand, Valiasr hospitals and Shariati dormitory. Generally, the impact of DEF was observed in the removing resistance more than PBO.

Conclusions: In most of these strains, resistance to bendiocarb and carbaryl is completely eliminated by DEF, showing a very high role of estraze enzymes in resistance to bendiocarb and carbaryl. But in most strains PBO does not remove the resistance because other mechanisms, such as reduced cuticle penetration and insensitivity to the acetylcholine esterase enzyme, may be involved.

Keywords: Bendiocarb; Blattella germanica; Carbaryl; Synergist.