Synthesizing efficacious genistein in conjugation with superparamagnetic Fe3O4 decorated with bio-compatible carboxymethylated chitosan against acute leukemia lymphoma

Rachel Ghasemi Goorbandi; Mohammad Reza Mohammadi; Kianoosh Malekzadeh

doi:10.1186/s40824-020-00187-2

Synthesizing efficacious genistein in conjugation with superparamagnetic Fe₃O₄ decorated with bio-compatible carboxymethylated chitosan against acute leukemia lymphoma

Biomater Res. 2020 Mar 20:24:9. doi: 10.1186/s40824-020-00187-2. eCollection 2020.

Authors

Rachel Ghasemi Goorbandi^{1

2}, Mohammad Reza Mohammadi³, Kianoosh Malekzadeh^{2

4}

Affiliations

¹ 1Sharif University of Technology, Kish International Campus, Kish Island, Iran.
² 2Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
³ 4Department of Medical Genetics; Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
⁴ 3Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran.

Abstract

Background: Genistein (C₁₅H₁₀O₅) is a soy isoflavone with anti-cancer properties such as inhibition of cell growth, proliferation and tumor invasion, but effective dosage against hematopoietic malignant cells was not in non-toxic range. This property cause to impede its usage as chemotherapeutic agent. Therefore, this hypothesis raised that synthesizing biocompatible nanoparticle could assist to prevail this struggle.

Methods: Genistein covalently attached on Fe₃O₄ nanoparticles decorated with carboxymethylated chitosan to fabricate Fe₃O₄-CMC-genistein in alkaline circumstance. This obtained nanoparticles were evaluated by TEM, DLS, FTIR, XRD and VSM and its anti-cancer effect by growth rate and MTT assays as well as flow cytometer on ALL cancer cell lines.

Results: Different evaluations indicated that the drug delivery vehicle had a mean diameter size around 12ƞm with well bounded components. This system presented high degree of magnetization and superparamagnetic properties as well as good water solubility. In comparison with pure genistein, significant growth inhibition on hematopoietic cancer cells in lower dose of genistein nano-conjugated onto Fe₃O₄-CMC. It increased long lasting effect of genistein in cancer cells also.

Conclusion: This delivery system for genistein could be remarkably promised and futuristic as biocompatible chemotherapeutic agent against hematopoietic malignant cells.

Keywords: Acute leukemia lymphoma (ALL); Chitosan; Genistein; Nano particle delivery system; Superparamagnetic Fe3O4.