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Synthesis of Fe-Doped Peroxidase Mimetic Nanozymes from Natural Hemoglobin for Colorimetric Biosensing and In Vitro Anticancer Effects.
Mohammadpour Z, Askari E, Shokati F, Hoseini HS, Kamankesh M, Zare Y, Rhee KY. Mohammadpour Z, et al. Biosensors (Basel). 2023 May 27;13(6):583. doi: 10.3390/bios13060583. Biosensors (Basel). 2023. PMID: 37366948 Free PMC article.
Particles that were prepared at 100 C (BDNP-100) showed the highest peroxidase mimetic activity, with Michaelis-Menten constants (K(m)) of 11.8 mM and 0.121 mM and maximum reaction rates (V(max)) of 8.56 10(-8) mol L(-1) s(-1) and 0.538 10(-8) mol L(-1) s(-1), for H(2 …
Particles that were prepared at 100 C (BDNP-100) showed the highest peroxidase mimetic activity, with Michaelis-Menten constants (K(m …
A Validated LC-MS/MS Assay for the Quantification of Cholate Isotopes in Human Serum.
Helmke SM, McRae MP, Christians U, Shokati T, Everson GT. Helmke SM, et al. J Appl Lab Med. 2024 Nov 4;9(6):1028-1039. doi: 10.1093/jalm/jfae094. J Appl Lab Med. 2024. PMID: 39150903
Lower and upper limits of quantitation were established and validated at 0.1 to 10.0 M. Matrix effects and potential interferents did not affect assay performance. DuO test validation met all prespecified acceptance criteria. ...
Lower and upper limits of quantitation were established and validated at 0.1 to 10.0 M. Matrix effects and potential interferents di …
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS.
Shokati T, Bodenberger N, Gadpaille H, Schniedewind B, Vinks AA, Jiang W, Alloway RR, Christians U. Shokati T, et al. J Vis Exp. 2015 Nov 8;(105):e52424. doi: 10.3791/52424. J Vis Exp. 2015. PMID: 26575262 Free PMC article.
The blood spot is homogenized using a bullet blender and then proteins are precipitated with methanol/0.2 M ZnSO4 containing the internal standard D2,(13)C-tacrolimus. After vortexing and centrifugation, 100 l of supernatant is injected into an online extraction column an …
The blood spot is homogenized using a bullet blender and then proteins are precipitated with methanol/0.2 M ZnSO4 containing the inte …
Simulated occlusal adjustments and their effects on zirconia and antagonist artificial enamel.
Alfrisany NM, Shokati B, Tam LE, De Souza GM. Alfrisany NM, et al. J Adv Prosthodont. 2019 Jun;11(3):162-168. doi: 10.4047/jap.2019.11.3.162. Epub 2019 Jun 26. J Adv Prosthodont. 2019. PMID: 31297175 Free PMC article.
MATERIALS AND METHODS: Twenty-five Y-TZP slabs from each brand (Lava, 3M and Bruxzir, Glidewell Laboratories) with different surface conditions (Control polished - CPZ; Polished/ground - GRZ; Polished/ground/repolished - RPZ; Glazed - GZ; Porcelain-veneered - PVZ; n=5) were abrad …
MATERIALS AND METHODS: Twenty-five Y-TZP slabs from each brand (Lava, 3M and Bruxzir, Glidewell Laboratories) with different surface conditi …
β-Caryophyllene Inhibits Monoacylglycerol Lipase Activity and Increases 2-Arachidonoyl Glycerol Levels In Vivo: A New Mechanism of Endocannabinoid-Mediated Analgesia?
Klawitter J, Weissenborn W, Gvon I, Walz M, Klawitter J, Jackson M, Sempio C, Joksimovic SL, Shokati T, Just I, Christians U, Todorovic SM. Klawitter J, et al. Mol Pharmacol. 2024 Jan 10;105(2):75-83. doi: 10.1124/molpharm.123.000668. Mol Pharmacol. 2024. PMID: 38195158 Free PMC article.
In vitro MAGL enzyme activity assessment using 2-AG as the substrate revealed an IC(50) of 15.8 M for MAGL inhibition using BCP. These data showed that BCP inhibits MAGL activity in vitro and in vivo, causing 2-AG levels to rise. ...
In vitro MAGL enzyme activity assessment using 2-AG as the substrate revealed an IC(50) of 15.8 M for MAGL inhibition using BCP. The …