The nucleotide-binding site of human sphingosine kinase 1.
Pitson SM, Moretti PA, Zebol JR, Zareie R, Derian CK, Darrow AL, Qi J, D'Andrea RJ, Bagley CJ, Vadas MA, Wattenberg BW.
Pitson SM, et al.
J Biol Chem. 2002 Dec 20;277(51):49545-53. doi: 10.1074/jbc.M206687200. Epub 2002 Oct 18.
J Biol Chem. 2002.
PMID: 12393916
Free article.
We have shown that Gly(82) of hSK1 is involved in ATP binding since mutation of this residue to alanine resulted in an enzyme with an approximately 45-fold higher K(m)((ATP)). We have also shown that Lys(103) is important in catalysis since an alanine substitution of this …
We have shown that Gly(82) of hSK1 is involved in ATP binding since mutation of this residue to alanine resulted in an enzyme with an approx …