Exploring roles of the chitinase ChiC in modulating Pseudomonas aeruginosa virulence phenotypes.
Edvardsen PKT, Askarian F, Zurich R, Nizet V, Vaaje-Kolstad G.
Edvardsen PKT, et al.
Microbiol Spectr. 2024 Jul 2;12(7):e0054624. doi: 10.1128/spectrum.00546-24. Epub 2024 May 31.
Microbiol Spectr. 2024.
PMID: 38819151
Free PMC article.
Enzyme kinetics toward (GlcNAc)(4) revealed a k(cat) of 6.50 s(-1) and a K(M) of 1.38 mM, the latter remarkably high for a canonical chitinase. In our label-free proteomics investigation, ChiC was among the most abundant proteins in the Pel biofilm, suggesting a potential …
Enzyme kinetics toward (GlcNAc)(4) revealed a k(cat) of 6.50 s(-1) and a K(M) of 1.38 mM, the latter remarkably high for a canonical …