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Page 1
A microRNA isolation method from clinical samples.
Zununi Vahed S, Barzegari A, Rahbar Saadat Y, Mohammadi S, Samadi N. Zununi Vahed S, et al. Bioimpacts. 2016;6(1):25-31. doi: 10.15171/bi.2016.04. Epub 2016 Mar 28. Bioimpacts. 2016. PMID: 27340621 Free PMC article.
RESULTS: Comparison of different RNA isolation methods based on the precipitation of DNA and large RNAs, high miRNA recovery and PCR efficiency revealed that applying potassium acetate with final precipitation of small RNAs using 2.5 M LiCl plus ethanol can provide high yi …
RESULTS: Comparison of different RNA isolation methods based on the precipitation of DNA and large RNAs, high miRNA recovery and PCR efficie …
Fabrication of mesoporous silica nanoparticles for targeted delivery of sunitinib to ovarian cancer cells.
Torabi M, Aghanejad A, Savadi P, Barzegari A, Omidi Y, Barar J. Torabi M, et al. Bioimpacts. 2023;13(3):255-267. doi: 10.34172/bi.2023.25298. Epub 2023 Jan 23. Bioimpacts. 2023. PMID: 37431477 Free PMC article.
RESULTS: The results revealed that the MSNPs have a spherical shape with an average dimension, pore size, and surface area of 56.10 nm, 2.488 nm, and 148.08 m(2)g(-1), respectively. The cell viability results showed higher toxicity of targeted MSNPs in MUC16 overexpressing …
RESULTS: The results revealed that the MSNPs have a spherical shape with an average dimension, pore size, and surface area of 56.10 nm, 2.48 …
Gene cloning and characterization of a novel recombinant 40-kDa heat shock protein from Mesobacillus persicus B48.
Fathinejad F, Ghafouri H, Barzegari E, Sarikhan S, Alizadeh A, Howard N. Fathinejad F, et al. World J Microbiol Biotechnol. 2023 Jul 12;39(9):248. doi: 10.1007/s11274-023-03693-2. World J Microbiol Biotechnol. 2023. PMID: 37436487
In addition, salt resistance experiments showed that the survival of recombinant E. coli+DnaJ was 2.1 times more than control cells in 0.5 M NaCl. Furthermore, the number of recombinant E. coli BL21+DnaJ colonies was 7.7 times that of the control colonies in pH 8.5. Based …
In addition, salt resistance experiments showed that the survival of recombinant E. coli+DnaJ was 2.1 times more than control cells in 0.5 …
The Impact of Terminal Peptide Extensions of Retinal Inosine 5´Monophosphate Dehydrogenase 1 Isoforms on their DNA-binding Activities.
Nabi Afjadi M, Yazdanparast R, Barzegari E. Nabi Afjadi M, et al. Protein J. 2024 Jun;43(3):592-602. doi: 10.1007/s10930-024-10202-3. Epub 2024 May 11. Protein J. 2024. PMID: 38733555
The overall impact of these predicted structural variations became evident, first, through higher K(m) values with respect to either of the substrates relative to the canonical isoform, as reported previously (Andashti et al. in Mol Cell Biochem 465(1):155-164, 2020). ...
The overall impact of these predicted structural variations became evident, first, through higher K(m) values with respect to either …
Zeolite Nanoparticles Inhibit Aβ-Fibrinogen Interaction and Formation of a Consequent Abnormal Structural Clot.
Derakhshankhah H, Hajipour MJ, Barzegari E, Lotfabadi A, Ferdousi M, Saboury AA, Ng EP, Raoufi M, Awala H, Mintova S, Dinarvand R, Mahmoudi M. Derakhshankhah H, et al. ACS Appl Mater Interfaces. 2016 Nov 16;8(45):30768-30779. doi: 10.1021/acsami.6b10941. Epub 2016 Nov 1. ACS Appl Mater Interfaces. 2016. PMID: 27766857
EMT-type zeolite nanoparticles (EMT NPs) with particle size of 10-20 nm and external surface area of 200 m(2)/g have shown high selective affinity toward plasma protein (fibrinogen). Besides, the EMT NPs have demonstrated no adverse effect on blood coagulation hemostasis. …
EMT-type zeolite nanoparticles (EMT NPs) with particle size of 10-20 nm and external surface area of 200 m(2)/g have shown high selec …
The functional impact of the C/N-terminal extensions of the mouse retinal IMPDH1 isoforms: a kinetic evaluation.
Andashti B, Yazdanparast R, Barzegari E, Galehdari H. Andashti B, et al. Mol Cell Biochem. 2020 Feb;465(1-2):155-164. doi: 10.1007/s11010-019-03675-9. Epub 2019 Dec 14. Mol Cell Biochem. 2020. PMID: 31838626
Molecular dynamic simulations indicated the possible impact of the end-terminal segments on the enzyme function through interactions with the enzyme's finger domain, affecting its critical pseudo barrel structure. The higher experimentally-determined K(m) and K(i) values o …
Molecular dynamic simulations indicated the possible impact of the end-terminal segments on the enzyme function through interactions with th …
Quinazolinone-dihydropyrano[3,2-b]pyran hybrids as new α-glucosidase inhibitors: Design, synthesis, enzymatic inhibition, docking study and prediction of pharmacokinetic.
Sherafati M, Mirzazadeh R, Barzegari E, Mohammadi-Khanaposhtani M, Azizian H, Sadegh Asgari M, Hosseini S, Zabihi E, Mojtabavi S, Ali Faramarzi M, Mahdavi M, Larijani B, Rastegar H, Hamedifar H, Hamed Hajimiri M. Sherafati M, et al. Bioorg Chem. 2021 Apr;109:104703. doi: 10.1016/j.bioorg.2021.104703. Epub 2021 Feb 8. Bioorg Chem. 2021. PMID: 33609917
Among them, the most potent compound was compound 10L (IC(50) value = 40.1 0.6 M) with inhibitory activity around 18.75-fold more than acarboase (IC(50) value = 750.0 12.5 M). ...
Among them, the most potent compound was compound 10L (IC(50) value = 40.1 0.6 M) with inhibitory activity around 18.75-fold more …
Introduction of a novel 18S rDNA gene arrangement along with distinct ITS region in the saline water microalga Dunaliella.
Hejazi MA, Barzegari A, Gharajeh NH, Hejazi MS. Hejazi MA, et al. Saline Syst. 2010 Apr 8;6:4. doi: 10.1186/1746-1448-6-4. Saline Syst. 2010. PMID: 20377865 Free PMC article.
The optimum growth rate of this isolate occured at the salinity level of 1 M NaCl. The maximum carotenoid content under stress condition of intense light (400 mumol photon m-2 s-1), high salinity (4 M NaCl) and deficiency of nitrate and phosphate nutritions r …
The optimum growth rate of this isolate occured at the salinity level of 1 M NaCl. The maximum carotenoid content under stress condit …