Menacho-Melgar R[au] - Search Results

Year	Number of Results
2017	1
2018	1
2019	1
2020	5
2021	2
2022	4

1

A review of lipidation in the development of advanced protein and peptide therapeutics.

Menacho-Melgar R, Decker JS, Hennigan JN, Lynch MD. Menacho-Melgar R, et al. J Control Release. 2019 Feb 10;295:1-12. doi: 10.1016/j.jconrel.2018.12.032. Epub 2018 Dec 21. J Control Release. 2019. PMID: 30579981 Free PMC article. Review.

2

Low-Cost, Large-Scale Production of the Anti-viral Lectin Griffithsin.

Decker JS, Menacho-Melgar R, Lynch MD. Decker JS, et al. Among authors: menacho melgar r. Front Bioeng Biotechnol. 2020 Aug 21;8:1020. doi: 10.3389/fbioe.2020.01020. eCollection 2020. Front Bioeng Biotechnol. 2020. PMID: 32974328 Free PMC article.

3

Simple Scalable Protein Expression and Extraction Using Two-stage Autoinducible Cell Autolysis and DNA/RNA Autohydrolysis in Escherichia coli.

Menacho-Melgar R, Lynch MD. Menacho-Melgar R, et al. Bio Protoc. 2022 Jan 20;12(2):e4297. doi: 10.21769/BioProtoc.4297. eCollection 2022 Jan 20. Bio Protoc. 2022. PMID: 35127987 Free article.

4

Optimization of phosphate-limited autoinduction broth for two-stage heterologous protein expression in Escherichia coli.

Menacho-Melgar R, Hennigan JN, Lynch MD. Menacho-Melgar R, et al. Biotechniques. 2021 Nov;71(5):566-572. doi: 10.2144/btn-2021-0055. Epub 2021 Aug 25. Biotechniques. 2021. PMID: 34431325 Free article.

5

Escherichia coli Cas1/2 Endonuclease Complex Modifies Self-Targeting CRISPR/Cascade Spacers Reducing Silencing Guide Stability.

Ye Z, Moreb EA, Li S, Lebeau J, Menacho-Melgar R, Munson M, Lynch MD. Ye Z, et al. Among authors: menacho melgar r. ACS Synth Biol. 2021 Jan 15;10(1):29-37. doi: 10.1021/acssynbio.0c00398. Epub 2020 Dec 17. ACS Synth Biol. 2021. PMID: 33331764

6

Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.

Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, Wang R, Lynch MD. Menacho-Melgar R, et al. Biotechnol Bioeng. 2020 Sep;117(9):2715-2727. doi: 10.1002/bit.27440. Epub 2020 Jun 10. Biotechnol Bioeng. 2020. PMID: 32441815

7

Media Robustness and Scalability of Phosphate Regulated Promoters Useful for Two-Stage Autoinduction in E. coli.

Moreb EA, Ye Z, Efromson JP, Hennigan JN, Menacho-Melgar R, Lynch MD. Moreb EA, et al. Among authors: menacho melgar r. ACS Synth Biol. 2020 Jun 19;9(6):1483-1486. doi: 10.1021/acssynbio.0c00182. Epub 2020 May 27. ACS Synth Biol. 2020. PMID: 32353228

8

Improved two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease.

Menacho-Melgar R, Moreb EA, Efromson JP, Yang T, Hennigan JN, Wang R, Lynch MD. Menacho-Melgar R, et al. Biotechnol Bioeng. 2020 Sep;117(9):2852-2860. doi: 10.1002/bit.27444. Epub 2020 Jun 20. Biotechnol Bioeng. 2020. PMID: 32462658

9

Measuring Oligonucleotide Hydrolysis in Cellular Lysates via Viscosity Measurements.

Menacho-Melgar R, Lynch MD. Menacho-Melgar R, et al. Bio Protoc. 2022 Jan 20;12(2):e4304. doi: 10.21769/BioProtoc.4304. eCollection 2022 Jan 20. Bio Protoc. 2022. PMID: 35127994 Free article.

10

Integrated autolysis, DNA hydrolysis and precipitation enables an improved bioprocess for Q-Griffithsin, a broad-spectrum antiviral and clinical-stage anti-COVID-19 candidate.

Decker JS, Menacho-Melgar R, Lynch MD. Decker JS, et al. Among authors: menacho melgar r. Biochem Eng J. 2022 Apr;181:108403. doi: 10.1016/j.bej.2022.108403. Epub 2022 Mar 12. Biochem Eng J. 2022. PMID: 35308834 Free PMC article.

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