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2019 3
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Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?
García-Ortega X, Cámara E, Ferrer P, Albiol J, Montesinos-Seguí JL, Valero F. García-Ortega X, et al. Among authors: montesinos segui jl. N Biotechnol. 2019 Nov 25;53:24-34. doi: 10.1016/j.nbt.2019.06.002. Epub 2019 Jun 10. N Biotechnol. 2019. PMID: 31195158 Review.
CPV of the Future: AI-Powered Continued Process Verification for Bioreactor Processes.
Ondracka A, Gasset A, García-Ortega X, Hubmayr D, van Wijngaarden J, Montesinos-Seguí JL, Valero F, Manzano T. Ondracka A, et al. Among authors: montesinos segui jl. PDA J Pharm Sci Technol. 2023 May-Jun;77(3):146-165. doi: 10.5731/pdajpst.2021.012665. Epub 2022 Sep 19. PDA J Pharm Sci Technol. 2023. PMID: 36122916
Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris.
Garrigós-Martínez J, Vuoristo K, Nieto-Taype MA, Tähtiharju J, Uusitalo J, Tukiainen P, Schmid C, Tolstorukov I, Madden K, Penttilä M, Montesinos-Seguí JL, Valero F, Glieder A, Garcia-Ortega X. Garrigós-Martínez J, et al. Among authors: montesinos segui jl. Microb Cell Fact. 2021 Mar 23;20(1):74. doi: 10.1186/s12934-021-01564-9. Microb Cell Fact. 2021. PMID: 33757505 Free PMC article.
Rationale-based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris).
Nieto-Taype MA, Garrigós-Martínez J, Sánchez-Farrando M, Valero F, Garcia-Ortega X, Montesinos-Seguí JL. Nieto-Taype MA, et al. Among authors: montesinos segui jl. Microb Biotechnol. 2020 Mar;13(2):315-327. doi: 10.1111/1751-7915.13498. Epub 2019 Oct 28. Microb Biotechnol. 2020. PMID: 31657146 Free PMC article.
Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage.
Garrigós-Martínez J, Nieto-Taype MA, Gasset-Franch A, Montesinos-Seguí JL, Garcia-Ortega X, Valero F. Garrigós-Martínez J, et al. Among authors: montesinos segui jl. Microb Cell Fact. 2019 Nov 1;18(1):187. doi: 10.1186/s12934-019-1240-8. Microb Cell Fact. 2019. PMID: 31675969 Free PMC article.