Kinetics of substrate utilization and bacterial growth of crude oil degraded by Pseudomonas aeruginosa

Amirreza Talaiekhozani; Nematollah Jafarzadeh; Mohamad Ali Fulazzaky; Mohammad Reza Talaie; Masoud Beheshti

doi:10.1186/s40201-015-0221-z

Kinetics of substrate utilization and bacterial growth of crude oil degraded by Pseudomonas aeruginosa

J Environ Health Sci Eng. 2015 Sep 24:13:64. doi: 10.1186/s40201-015-0221-z. eCollection 2015.

Authors

Amirreza Talaiekhozani¹, Nematollah Jafarzadeh², Mohamad Ali Fulazzaky³, Mohammad Reza Talaie⁴, Masoud Beheshti⁵

Affiliations

¹ Department of Civil and Environmental Engineering, Jami Institute of Technology, Isfahan, Iran ; Centre for Environmental Sustainability and Water Security, Research Institute for Sustainable Environment, Universiti Teknologi Malaysia, 81310 UTM Skudai, Bahru, Johor Malaysia.
² Department of Environmental Health, School of Health, Jondishapour University of Medical Science, Ahwaz, Iran.
³ Centre for Environmental Sustainability and Water Security, Research Institute for Sustainable Environment, Universiti Teknologi Malaysia, 81310 UTM Skudai, Bahru, Johor Malaysia ; Faculty of Civil Engineering, Universiti Teknologi Malaysia, Bahru, Johor Malaysia.
⁴ Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Kuala Lumpur, Malaysia ; Department of Chemical Engineering, Isfahan University, Isfahan, Iran.
⁵ Department of Chemical Engineering, Isfahan University, Isfahan, Iran.

Abstract

Pollution associated with crude oil (CO) extraction degrades the quality of waters, threatens drinking water sources and may ham air quality. The systems biology approach aims at learning the kinetics of substrate utilization and bacterial growth for a biological process for which very limited knowledge is available. This study uses the Pseudomonas aeruginosa to degrade CO and determines the kinetic parameters of substrate utilization and bacterial growth modeled from a completely mixed batch reactor. The ability of Pseudomonas aeruginosa can remove 91 % of the total petroleum hydrocarbons and 83 % of the aromatic compounds from oily environment. The value k of 9.31 g of substrate g(-1) of microorganism d(-1) could be far higher than the value k obtained for petrochemical wastewater treatment and that for municipal wastewater treatment. The production of new cells of using CO as the sole carbon and energy source can exceed 2(3) of the existing cells per day. The kinetic parameters are verified to contribute to improving the biological removal of CO from oily environment.

Keywords: Bacterial growth; Crude oil; Kinetic model; Pseudomonas aeruginosa; Substrate utilization.