Brucellosis is known as an important public health problem for humans around the world and is one of the most prevalent zoonotic disease as well. Brucella spp. is a Gram negative rod, facultative intracellular pathogenic and non motile bacteria. The aim of this study were to determine the frequency and biotyping of Brucella spp. isolated from sheep and goats from south eastern part of Iran. 700 samples (raw milk) from sheep and goats (90) flocks were collected randomly, from January to July 2015, samples were transferred packed in ice to microbiology lab in Kerman University, immediately. Samples were inoculated on Brucella agar which contains antibiotic and inactivated horse serum and incubate for 5 days. Gram smears were prepared from suspected colonies followed by oxidize and biochemical tests. Polymerase Chain Reaction (PCR) were used for confirmation. All positive samples were biotyped by phage typing. Nine milk samples out of 700 (1.28%) collected were positive by bacteriological method and all of them were Brucella melitensis Biotype 1 and one out of 700 samples was Brucella ovis. In relation to the National vaccination program in these areas the most contaminated region was Rabor, Rayen, Bardsir and Baft, respectively.