Survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold

Sajad Sahab Negah; Hadi Aligholi; Zabihollah Khaksar; Hadi Kazemi; Sayed Mostafa Modarres Mousavi; Maryam Safahani; Parastoo Barati Dowom; Ali Gorji

doi:10.22038/ijbms.2016.7907

Survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold

Iran J Basic Med Sci. 2016 Dec;19(12):1271-1278. doi: 10.22038/ijbms.2016.7907.

Authors

Sajad Sahab Negah¹, Hadi Aligholi², Zabihollah Khaksar³, Hadi Kazemi⁴, Sayed Mostafa Modarres Mousavi⁵, Maryam Safahani⁶, Parastoo Barati Dowom⁵, Ali Gorji⁷

Affiliations

¹ Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran; Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
² Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
³ Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.
⁴ Pediatric Department, Medical Faculty, Shahed University, Tehran, Iran.
⁵ Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
⁶ Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran; Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
⁷ Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran; Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Germany; Department of Neurology and Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Germany; Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract

Objectives: In order to grow cells in a three-dimensional (3D) microenvironment, self-assembling peptides, such as PuraMatrix, have emerged with potential to mimic the extracellular matrix. The aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma stem-like cells (hMgSCs).

Materials and methods: The efficacy of a novel method for placing hMgSCs in PuraMatrix (the injection approach) was compared to the encapsulation and surface plating methods. In addition, we designed a new method for measurement of migration distance in 3D cultivation of hMgSCs in PuraMatrix.

Results: Our results revealed that hMgSCs have the ability to form spheres in stem cell culture condition. These meningioma cells expressed GFAP, CD133, vimentin, and nestin. Using the injection method, a higher proliferation rate of the hMgSCs was observed after seven days of culture. Furthermore, the novel migration assay was able to measure the migration of a single cell alone in 3D environment.

Conclusion: The results indicate the injection method as an efficient technique for culturing hMgSCs in PuraMatrix. Furthermore, the novel migration assay enables us to evaluate the migration of hMgSCs.

Keywords: Brain tumor; Cell culture; Cell migration; RADA16-I; Stem cells.