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R-loop formation and conformational activation mechanisms of Cas9.
Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M. Pacesa M, et al. Among authors: querques i. Nature. 2022 Sep;609(7925):191-196. doi: 10.1038/s41586-022-05114-0. Epub 2022 Aug 24. Nature. 2022. PMID: 36002571 Free PMC article.
A highly soluble Sleeping Beauty transposase improves control of gene insertion.
Querques I, Mades A, Zuliani C, Miskey C, Alb M, Grueso E, Machwirth M, Rausch T, Einsele H, Ivics Z, Hudecek M, Barabas O. Querques I, et al. Nat Biotechnol. 2019 Dec;37(12):1502-1512. doi: 10.1038/s41587-019-0291-z. Epub 2019 Nov 4. Nat Biotechnol. 2019. PMID: 31685959 Free PMC article.
A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming.
Kesselring L, Miskey C, Zuliani C, Querques I, Kapitonov V, Laukó A, Fehér A, Palazzo A, Diem T, Lustig J, Sebe A, Wang Y, Dinnyés A, Izsvák Z, Barabas O, Ivics Z. Kesselring L, et al. Among authors: querques i. Nucleic Acids Res. 2020 Jan 10;48(1):316-331. doi: 10.1093/nar/gkz1119. Nucleic Acids Res. 2020. PMID: 31777924 Free PMC article.