Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues.
Glaser A, Chandrashekar J, Vasquez S, Arshadi C, Javeri R, Ouellette N, Jiang X, Baka J, Kovacs G, Woodard M, Seshamani S, Cao K, Clack N, Recknagel A, Grim A, Balaram P, Turschak E, Hooper M, Liddell A, Rohde J, Hellevik A, Takasaki K, Erion Barner L, Logsdon M, Chronopoulos C, de Vries SEJ, Ting JT, Perlmutter S, Kalmbach BE, Dembrow N, Tasic B, Reid RC, Feng D, Svoboda K.
Glaser A, et al.
Elife. 2025 Jun 30;12:RP91979. doi: 10.7554/eLife.91979.
Elife. 2025.
PMID: 40586775
Free PMC article.
We present a new expansion-assisted selective plane illumination microscope (ExA-SPIM) with aberration-free 1.5 m 1.5 m 3 m optical resolution over a large field of view (10.6 8.0 mm(2)) and working distance (35 mm) at speeds up to 946 megavoxels/s. Combined with new ti …
We present a new expansion-assisted selective plane illumination microscope (ExA-SPIM) with aberration-free 1.5 m 1.5 m 3 m optical resol …