Data on cell survival, apoptosis, ceramide metabolism and oxidative stress in A-494 renal cell carcinoma cell line treated with hesperetin and hesperetin-7-O-acetate

Mahdi Mashhadi Akbar Boojar; Masoud Mashhadi Akbar Boojar; Sepide Golmohammad; Iraj Bahrehbar

doi:10.1016/j.dib.2018.08.065

Data on cell survival, apoptosis, ceramide metabolism and oxidative stress in A-494 renal cell carcinoma cell line treated with hesperetin and hesperetin-7-O-acetate

Data Brief. 2018 Aug 29:20:596-601. doi: 10.1016/j.dib.2018.08.065. eCollection 2018 Oct.

Authors

Mahdi Mashhadi Akbar Boojar^{1

2}, Masoud Mashhadi Akbar Boojar³, Sepide Golmohammad³, Iraj Bahrehbar³

Affiliations

¹ Department of Pharmacology and Toxicology, Baqiyatallah University of Medical Sciences, Tehran, Iran.
² Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.
³ Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.

Abstract

Ceramide pathway is a key regulator in cell proliferation and apoptosis and oxidative stress up-regulate ceramides. Acid ceramidase (ACDase), neutral sphingomyelinase (NSMase) and glucosylceramide synthase (GCS) are critical enzymatic systems in ceramide metabolism. Our data represent the comparative assessment of Hesperetin (Hst) and hesperetin-7-O-acetate (HTA) effects on A-494 renal carcinoma cells include cell survival, caspase-3 and 9 activities, total cellular ceramide and the activities of ACDase, NSMase, GCS and superoxide dismutase (SOD). Data reveals potentiating effects of both HTA and Hst on ceramide pathway and may offer a novel tool in human renal cell carcinoma therapy.

Keywords: Ceramidase; Ceramide; Hesperetin; Sphingomyelinase; Superoxide dismutase.