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Showing results for vitro dudek j[au]
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Loss of Mitochondrial Ca2+ Uniporter Limits Inotropic Reserve and Provides Trigger and Substrate for Arrhythmias in Barth Syndrome Cardiomyopathy.
Bertero E, Nickel A, Kohlhaas M, Hohl M, Sequeira V, Brune C, Schwemmlein J, Abeßer M, Schuh K, Kutschka I, Carlein C, Münker K, Atighetchi S, Müller A, Kazakov A, Kappl R, von der Malsburg K, van der Laan M, Schiuma AF, Böhm M, Laufs U, Hoth M, Rehling P, Kuhn M, Dudek J, von der Malsburg A, Prates Roma L, Maack C. Bertero E, et al. Among authors: dudek j. Circulation. 2021 Nov 23;144(21):1694-1713. doi: 10.1161/CIRCULATIONAHA.121.053755. Epub 2021 Oct 14. Circulation. 2021. PMID: 34648376 Free article.
All alterations occurred in the absence of excess mitochondrial ROS in vitro or in vivo. CONCLUSIONS: Downregulation of mitochondrial Ca(2+) uniporter, increased myofilament Ca(2+) affinity, and preactivated sarcoplasmic reticulum Ca(2+)-ATPase provoke mechano-energetic un …
All alterations occurred in the absence of excess mitochondrial ROS in vitro or in vivo. CONCLUSIONS: Downregulation of mitochondrial …
LDL dinitrosyl iron complex acts as an iron donor in mouse macrophages.
Lewandowska H, Stępkowski TM, Męczyńska-Wielgosz S, Sikorska K, Sadło J, Dudek J, Kruszewski M. Lewandowska H, et al. Among authors: dudek j. J Inorg Biochem. 2018 Nov;188:29-37. doi: 10.1016/j.jinorgbio.2018.08.004. Epub 2018 Aug 4. J Inorg Biochem. 2018. PMID: 30119015
High increase of iron was accompanied by moderate toxicity. As shown by in vitro plasmid nicking assay, chelation of iron in the form of DNIC strongly reduced the iron-related reactive oxygen species (ROS) -induced DNA damage. ...
High increase of iron was accompanied by moderate toxicity. As shown by in vitro plasmid nicking assay, chelation of iron in the form …
Genetic Ablation of the Mitochondrial Calcium Uniporter (MCU) Does not Impair T Cell-Mediated Immunity In Vivo.
Wu H, Brand B, Eckstein M, Hochrein SM, Shumanska M, Dudek J, Nickel A, Maack C, Bogeski I, Vaeth M. Wu H, et al. Among authors: dudek j. Front Pharmacol. 2021 Dec 20;12:734078. doi: 10.3389/fphar.2021.734078. eCollection 2021. Front Pharmacol. 2021. PMID: 34987384 Free PMC article.
However, ablation of MCU and the elevation of cytosolic Ca(2+) did not affect mitochondrial respiration, differentiation and effector function of inflammatory and regulatory T cell subsets in vitro and in animal models of T cell-mediated autoimmunity and viral infection. . …
However, ablation of MCU and the elevation of cytosolic Ca(2+) did not affect mitochondrial respiration, differentiation and effector functi …
Analysis of protein translocation into the endoplasmic reticulum of human cells.
Dudek J, Lang S, Schorr S, Linxweiler J, Greiner M, Zimmermann R. Dudek J, et al. Methods Mol Biol. 2013;1033:285-99. doi: 10.1007/978-1-62703-487-6_18. Methods Mol Biol. 2013. PMID: 23996184
In these approaches, a certain target gene is silenced in the cell type of choice, followed by analysis of the effect of this silencing on the biogenesis of a single or set of precursor polypeptide(s) in cell culture or in cell-free assays involving semi-permeabilized cells and i …
In these approaches, a certain target gene is silenced in the cell type of choice, followed by analysis of the effect of this silencing on t …
Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import.
Schorr S, Nguyen D, Haßdenteufel S, Nagaraj N, Cavalié A, Greiner M, Weissgerber P, Loi M, Paton AW, Paton JC, Molinari M, Förster F, Dudek J, Lang S, Helms V, Zimmermann R. Schorr S, et al. Among authors: dudek j. FEBS J. 2020 Nov;287(21):4612-4640. doi: 10.1111/febs.15274. Epub 2020 Mar 20. FEBS J. 2020. PMID: 32133789 Free article.
So far, mainly posttranslationally imported precursors and the two cotranslationally imported precursors of ERj3 and prion protein were found to depend on the Sec62/Sec63 complex in vitro. Therefore, we determined the rules for engagement of Sec62/Sec63 in ER import in int …
So far, mainly posttranslationally imported precursors and the two cotranslationally imported precursors of ERj3 and prion protein were foun …
Alphavirus replicon approach to promoterless analysis of IRES elements.
Kamrud KI, Custer M, Dudek JM, Owens G, Alterson KD, Lee JS, Groebner JL, Smith JF. Kamrud KI, et al. Among authors: dudek jm. Virology. 2007 Apr 10;360(2):376-87. doi: 10.1016/j.virol.2006.10.049. Epub 2006 Dec 6. Virology. 2007. PMID: 17156813 Free PMC article.
Alphavirus replicons, used in this manner, offer an alternative to standard dicistronic DNA vectors or in vitro translation systems currently used to analyze putative IRES elements. ...
Alphavirus replicons, used in this manner, offer an alternative to standard dicistronic DNA vectors or in vitro translation systems c …
Development and characterization of promoterless helper RNAs for the production of alphavirus replicon particle.
Kamrud KI, Alterson K, Custer M, Dudek J, Goodman C, Owens G, Smith JF. Kamrud KI, et al. Among authors: dudek j. J Gen Virol. 2010 Jul;91(Pt 7):1723-7. doi: 10.1099/vir.0.020081-0. Epub 2010 Feb 24. J Gen Virol. 2010. PMID: 20181749 Free PMC article.
Typically, alphavirus replicon RNAs are delivered within virus-like replicon particles (VRP) that are produced following transfection of replicon RNA and two helper RNAs into permissive cells in vitro. The non-structural proteins expressed from the replicon RNA amplify the …
Typically, alphavirus replicon RNAs are delivered within virus-like replicon particles (VRP) that are produced following transfection of rep …
The presequence translocase-associated protein import motor of mitochondria. Pam16 functions in an antagonistic manner to Pam18.
Li Y, Dudek J, Guiard B, Pfanner N, Rehling P, Voos W. Li Y, et al. Among authors: dudek j. J Biol Chem. 2004 Sep 3;279(36):38047-54. doi: 10.1074/jbc.M404319200. Epub 2004 Jun 24. J Biol Chem. 2004. PMID: 15218029 Free article.
The inclusion of the HPD motif in Pam16 does not confer the ability to stimulate mtHsp70 activity. Pam16-HPD fully substitutes for wild-type Pam16 in vitro and in vivo but is not able to replace Pam18. Pam16 represents a new type of cochaperone that controls the stimulator …
The inclusion of the HPD motif in Pam16 does not confer the ability to stimulate mtHsp70 activity. Pam16-HPD fully substitutes for wild-type …
Cardiolipin deficiency affects respiratory chain function and organization in an induced pluripotent stem cell model of Barth syndrome.
Dudek J, Cheng IF, Balleininger M, Vaz FM, Streckfuss-Bömeke K, Hübscher D, Vukotic M, Wanders RJ, Rehling P, Guan K. Dudek J, et al. Stem Cell Res. 2013 Sep;11(2):806-19. doi: 10.1016/j.scr.2013.05.005. Epub 2013 May 28. Stem Cell Res. 2013. PMID: 23792436 Free article.
BTHS-iPSCs generated from dermal fibroblasts of three patients with different mutations in TAZ1 expressed pluripotency markers, and were able to differentiate into cells derived from all three germ layers both in vitro and in vivo. We used these cells to study the impact o …
BTHS-iPSCs generated from dermal fibroblasts of three patients with different mutations in TAZ1 expressed pluripotency markers, and were abl …