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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1986 1
1991 2
1992 2
1993 6
1994 2
1995 5
1996 23
1997 25
1998 42
1999 138
2000 398
2001 804
2002 1565
2003 2566
2004 3734
2005 5170
2006 6361
2007 7192
2008 8135
2009 8862
2010 10128
2011 13560
2012 17814
2013 18476
2014 18289
2015 18586
2016 16839
2017 15427
2018 15096
2019 15436
2020 13380
2021 26
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190,786 results
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Page 1
Multiplex Analyses Using Real-Time Quantitative PCR.
Hawkins SF, Guest PC. Hawkins SF, et al. Methods Mol Biol. 2017;1546:125-133. doi: 10.1007/978-1-4939-6730-8_8. Methods Mol Biol. 2017. PMID: 27896761 Review.
Quantitative polymerase chain reaction (qPCR) is a routinely used method for the detection and quantitation of gene expression in real time. ...Here, we describe a method using the SensiFAST and SensiFAST One-Step probe kits which allows simulta …
Quantitative polymerase chain reaction (qPCR) is a routinely used method for the detection and quantitation of gene exp …
Quantitative Real-Time PCR: Recent Advances.
Singh C, Roy-Chowdhuri S. Singh C, et al. Methods Mol Biol. 2016;1392:161-76. doi: 10.1007/978-1-4939-3360-0_15. Methods Mol Biol. 2016. PMID: 26843055
Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a "closed-tube" system. ...The quantification proc …
Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and produc …
PCR-Based Detection Methods for Single-Nucleotide Polymorphism or Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological Refinement.
Matsuda K. Matsuda K. Adv Clin Chem. 2017;80:45-72. doi: 10.1016/bs.acc.2016.11.002. Epub 2017 Jan 4. Adv Clin Chem. 2017. PMID: 28431642 Review.
In this regard, polymerase chain reaction (PCR) has provided the necessary analytical performance for many molecular analyses. PCR-based methods for SNP/mutation detection are broadly categorized into two types-(1) polymorphic or mutant allele-d …
In this regard, polymerase chain reaction (PCR) has provided the necessary analytical performance for many molec …
Democratizing the Real-Time PCR.
Wittwer CT. Wittwer CT. Clin Chem. 2017 Apr;63(4):924-925. doi: 10.1373/clinchem.2016.263269. Epub 2017 Feb 3. Clin Chem. 2017. PMID: 28159801 No abstract available.
Analysis and Normalization of Real-Time Polymerase Chain Reaction (PCR) Experimental Data.
Green MR, Sambrook J. Green MR, et al. Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.top095000. Cold Spring Harb Protoc. 2018. PMID: 30275081
In real-time polymerase chain reaction (PCR), also called quantitative real-time PCR [or simply quantitative PCR (qPCR)] or kinetic PCR, the amplification of DNA is monitored by the detection and quantit …
In real-time polymerase chain reaction (PCR), also called quantitative real-time
Minimum information necessary for quantitative real-time PCR experiments.
Johnson G, Nour AA, Nolan T, Huggett J, Bustin S. Johnson G, et al. Methods Mol Biol. 2014;1160:5-17. doi: 10.1007/978-1-4939-0733-5_2. Methods Mol Biol. 2014. PMID: 24740217
The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines were published in 2009 with the twin aims of providing a blueprint for good real-time quantitative polymerase chain reaction (qPCR) …
The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines were published in 2009 with …
Overcoming inhibition in real-time diagnostic PCR.
Hedman J, Rådström P. Hedman J, et al. Methods Mol Biol. 2013;943:17-48. doi: 10.1007/978-1-60327-353-4_2. Methods Mol Biol. 2013. PMID: 23104280
In addition, the thermostable DNA polymerases and buffer systems used in PCR are affected differently by inhibitors. During recent years, real-time PCR has developed considerably and is now widely used as a diagnostic tool. ...The concept of integratin …
In addition, the thermostable DNA polymerases and buffer systems used in PCR are affected differently by inhibitors. During recent ye …
Real-time PCR assays for diagnosing brucellar spondylitis using formalin-fixed paraffin-embedded tissues.
Li M, Zhou X, Li J, Sun L, Chen X, Wang P. Li M, et al. Medicine (Baltimore). 2018 Mar;97(9):e0062. doi: 10.1097/MD.0000000000010062. Medicine (Baltimore). 2018. PMID: 29489665 Free PMC article.
This study aimed to determine whether real-time polymerase chain reaction (PCR) using formalin-fixed paraffin-embedded (FFPE) tissues was superior to conventional serum-based methods for diagnosing brucellar spondylitis.This retrospective …
This study aimed to determine whether real-time polymerase chain reaction (PCR) using formalin-fix …
Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR).
Green MR, Sambrook J. Green MR, et al. Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095034. Cold Spring Harb Protoc. 2018. PMID: 30275076
There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and a standard polymerase chain reaction (PCR). In real-time PCR, it is necessary, however, to …
There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and …
A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses.
Shi X, Liu X, Wang Q, Das A, Ma G, Xu L, Sun Q, Peddireddi L, Jia W, Liu Y, Anderson G, Bai J, Shi J. Shi X, et al. J Virol Methods. 2016 Oct;236:258-265. doi: 10.1016/j.jviromet.2016.08.005. Epub 2016 Aug 6. J Virol Methods. 2016. PMID: 27506582 Free PMC article.
Correlation coefficients (R(2)) and PCR amplification efficiencies of all singular and triplex real-time PCR reactions are within the acceptable range. ...The limit of detection of each triplex real-time PCR is less than 10 copies …
Correlation coefficients (R(2)) and PCR amplification efficiencies of all singular and triplex real-time PCR rea …
190,786 results
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