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1991 1
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Assay of pyrogens by interleukin-6 release from monocytic cell lines.
Taktak YS, Selkirk S, Bristow AF, Carpenter A, Ball C, Rafferty B, Poole S. Taktak YS, et al. J Pharm Pharmacol. 1991 Aug;43(8):578-82. doi: 10.1111/j.2042-7158.1991.tb03540.x. J Pharm Pharmacol. 1991. PMID: 1681074
A novel in-vitro system has been developed for the detection and quantification of pyrogen in pharmaceutical products. The measured variable was evoked secretion of the pyrogenic cytokine interleukin-6 from MONO MAC 6 monocytic cells incu …
A novel in-vitro system has been developed for the detection and quantification of pyrogen in pharmaceutical products. The measured v …
A rapid 'one-plate' in vitro test for pyrogens.
Poole S, Mistry Y, Ball C, Gaines Das RE, Opie LP, Tucker G, Patel M. Poole S, et al. J Immunol Methods. 2003 Mar 1;274(1-2):209-20. doi: 10.1016/s0022-1759(02)00519-7. J Immunol Methods. 2003. PMID: 12609546
Monocytes/monocytic cells, endotoxin (lipopolysaccharides, LPS) standard or sample and (pyrogen-free) second (labelled) Ab are cultured together (usually for 2-4 h) on the Ab-coated plate and then the plate is washed and the ELISA completed. ...The rapid, 'on
Monocytes/monocytic cells, endotoxin (lipopolysaccharides, LPS) standard or sample and (pyrogen-free) second (labelled)
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