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By CyTOF: Heterogeneity of Human Monocytes.
Loke P, Niewold TB. Loke P, et al. Arterioscler Thromb Vasc Biol. 2017 Aug;37(8):1423-1424. doi: 10.1161/ATVBAHA.117.309645. Arterioscler Thromb Vasc Biol. 2017. PMID: 28747454 Free PMC article.
Human monocyte subsets have traditionally been defined based on expression of CD14 and CD16 into classical, non-classical and intermediate monocytes. Using CyTOF, Thomas et.al. identified CCR2, CD36, HLA-DR and CD11c as additional cell surface markers
Human monocyte subsets have traditionally been defined based on expression of CD14 and CD16 into classical, non-classical and
Mass cytometry deep phenotyping of human mononuclear phagocytes and myeloid-derived suppressor cells from human blood and bone marrow.
Roussel M, Ferrell PB Jr, Greenplate AR, Lhomme F, Le Gallou S, Diggins KE, Johnson DB, Irish JM. Roussel M, et al. J Leukoc Biol. 2017 Aug;102(2):437-447. doi: 10.1189/jlb.5MA1116-457R. Epub 2017 Apr 11. J Leukoc Biol. 2017. PMID: 28400539 Free PMC article.
The monocyte phagocyte system (MPS) includes numerous monocyte, macrophage, and dendritic cell (DC) populations that are heterogeneous, both phenotypically and functionally. ...In summary, mass cytometry comprehensively characterized phenotypes of human
The monocyte phagocyte system (MPS) includes numerous monocyte, macrophage, and dendritic cell (DC) populations that are he
Single-Cell Mapping of Human Brain Cancer Reveals Tumor-Specific Instruction of Tissue-Invading Leukocytes.
Friebel E, Kapolou K, Unger S, Núñez NG, Utz S, Rushing EJ, Regli L, Weller M, Greter M, Tugues S, Neidert MC, Becher B. Friebel E, et al. Cell. 2020 Jun 25;181(7):1626-1642.e20. doi: 10.1016/j.cell.2020.04.055. Epub 2020 May 28. Cell. 2020. PMID: 32470397
Here, we have mapped the leukocyte landscape of brain tumors using high-dimensional single-cell profiling (CyTOF). The heterogeneous composition of tissue-resident and invading immune cells within the TME alone permitted a clear distinction between gliomas and brain …
Here, we have mapped the leukocyte landscape of brain tumors using high-dimensional single-cell profiling (CyTOF). The heterogeneo
A Novel, Five-Marker Alternative to CD16-CD14 Gating to Identify the Three Human Monocyte Subsets.
Ong SM, Teng K, Newell E, Chen H, Chen J, Loy T, Yeo TW, Fink K, Wong SC. Ong SM, et al. Front Immunol. 2019 Jul 26;10:1761. doi: 10.3389/fimmu.2019.01761. eCollection 2019. Front Immunol. 2019. PMID: 31402918 Free PMC article.
Human primary monocytes are heterogeneous in terms of phenotype and function, but are sub-divided only based on CD16 and CD14 expression. ...Using cytometry by time-of-flight (CyTOF), we studied the simultaneous expression of 34 monocyte markers
Human primary monocytes are heterogeneous in terms of phenotype and function, but are sub-divided only based on CD16 an
Candidate Markers for Stratification and Classification in Rheumatoid Arthritis.
Bader L, Gullaksen SE, Blaser N, Brun M, Bringeland GH, Sulen A, Gjesdal CG, Vedeler C, Gavasso S. Bader L, et al. Front Immunol. 2019 Jul 5;10:1488. doi: 10.3389/fimmu.2019.01488. eCollection 2019. Front Immunol. 2019. PMID: 31338093 Free PMC article.
RA is a heterogeneous disease with a plethora of treatment options. The pro-inflammatory cytokine tumor necrosis factor (TNF) plays a central role in the pathogenesis of RA, and TNF inhibitors effectively repress inflammatory activity in RA. ...We employed mass cytometry ( …
RA is a heterogeneous disease with a plethora of treatment options. The pro-inflammatory cytokine tumor necrosis factor (TNF) plays a …
Individual liver plasmacytoid dendritic cells are capable of producing IFNα and multiple additional cytokines during chronic HCV infection.
Doyle EH, Rahman A, Aloman C, Klepper AL, El-Shamy A, Eng F, Rocha C, Kim S, Haydel B, Florman SS, Fiel MI, Schiano T, Branch AD. Doyle EH, et al. PLoS Pathog. 2019 Jul 29;15(7):e1007935. doi: 10.1371/journal.ppat.1007935. eCollection 2019 Jul. PLoS Pathog. 2019. PMID: 31356648 Free PMC article.
Mass cytometry (CyTOF) revealed that liver pDCs are the only LMC that produces detectable amounts of IFNα in response TLR-7/8 stimulation. ...Liver pDC frequency positively correlated with whole liver expression of "IFNα-response" pathway (R2 = 0.58, p = 0.007) and "mon
Mass cytometry (CyTOF) revealed that liver pDCs are the only LMC that produces detectable amounts of IFNα in response TLR-7/8 stimula …
Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans.
Cytlak U, Resteu A, Pagan S, Green K, Milne P, Maisuria S, McDonald D, Hulme G, Filby A, Carpenter B, Queen R, Hambleton S, Hague R, Lango Allen H, Thaventhiran JED, Doody G, Collin M, Bigley V. Cytlak U, et al. Immunity. 2020 Aug 18;53(2):353-370.e8. doi: 10.1016/j.immuni.2020.07.003. Epub 2020 Jul 30. Immunity. 2020. PMID: 32735845 Free PMC article.
In humans, cDC2-related subsets have been described including AXL(+)SIGLEC6(+) pre-DC, DC2 and DC3. The origin of this heterogeneity is unknown. Using high-dimensional analysis, in vitro differentiation, and an allelic series of human IRF8 deficiency, we demo …
In humans, cDC2-related subsets have been described including AXL(+)SIGLEC6(+) pre-DC, DC2 and DC3. The origin of this heterogenei
Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry.
Roussel M, Bartkowiak T, Irish JM. Roussel M, et al. Methods Mol Biol. 2019;1989:217-226. doi: 10.1007/978-1-4939-9454-0_14. Methods Mol Biol. 2019. PMID: 31077108 Free PMC article.
The immune monocyte/phagocyte system (MPS) includes numerous cell subsets of the myeloid lineage including monocyte, macrophage, and dendritic cell (DC) populations that are heterogeneous both phenotypically and functionally. Previously, we characterized thes …
The immune monocyte/phagocyte system (MPS) includes numerous cell subsets of the myeloid lineage including monocyte, macrophag …
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