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Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis.
Marimuthu R, Francis H, Dervish S, Li SCH, Medbury H, Williams H. Marimuthu R, et al. J Vis Exp. 2018 Oct 17;(140):57941. doi: 10.3791/57941. J Vis Exp. 2018. PMID: 30394370 Free PMC article.
An ideal method for examining alterations to monocytes is whole blood flow cytometry as the minimal handling of samples by this method limits artifactual cell activation. ...Here we demonstrate a method using whole blood flow
An ideal method for examining alterations to monocytes is whole blood flow cytometry as the minimal handl …
OMIP-94: Twenty-four-color (thirty-marker) panel for deep immunophenotyping of immune cells in human peripheral blood.
Pierzchalski A, Zenclussen AC, Herberth G. Pierzchalski A, et al. Cytometry A. 2023 Sep;103(9):695-702. doi: 10.1002/cyto.a.24766. Epub 2023 May 30. Cytometry A. 2023. PMID: 37254600
This newly established 24-color (30-marker) panel focuses on the characterization of the main human immune cell subtypes and was optimized for the analysis of human whole blood using a full spectrum flow cytometer. ...The presented …
This newly established 24-color (30-marker) panel focuses on the characterization of the main human immune cell subtypes and w …
Standardisation of flow cytometry for whole blood immunophenotyping of islet transplant and transplant clinical trial recipients.
Jimenez Vera E, Chew YV, Nicholson L, Burns H, Anderson P, Chen HT, Williams L, Keung K, Zanjani NT, Dervish S, Patrick E, Wang XM, Yi S, Hawthorne W, Alexander S, O'Connell PJ, Hu M. Jimenez Vera E, et al. PLoS One. 2019 May 22;14(5):e0217163. doi: 10.1371/journal.pone.0217163. eCollection 2019. PLoS One. 2019. PMID: 31116766 Free PMC article.
Understanding the immunological phenotype of transplant recipients is important to improve outcomes and develop new therapies. Immunophenotyping of whole peripheral blood (WPB) by flow cytometry is a rapid method to obtain large amounts of data relatin …
Understanding the immunological phenotype of transplant recipients is important to improve outcomes and develop new therapies. Immunophenoty …
Characterization of a whole blood assay for quantifying myeloid-derived suppressor cells.
Apodaca MC, Wright AE, Riggins AM, Harris WP, Yeung RS, Yu L, Morishima C. Apodaca MC, et al. J Immunother Cancer. 2019 Aug 28;7(1):230. doi: 10.1186/s40425-019-0674-1. J Immunother Cancer. 2019. PMID: 31462270 Free PMC article.
Optimal measurement of MDSC levels could provide clinicians with a useful prognostic and/or management tool. METHODS: A whole blood (WB) nine color, 11 parameter flow cytometric assay was designed, utilizing fluorescently-labeled antibodies against CD45, CD3, …
Optimal measurement of MDSC levels could provide clinicians with a useful prognostic and/or management tool. METHODS: A whole bloo
Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population Discovery.
Baumgaertner P, Sankar M, Herrera F, Benedetti F, Barras D, Thierry AC, Dangaj D, Kandalaft LE, Coukos G, Xenarios I, Guex N, Harari A. Baumgaertner P, et al. Front Immunol. 2021 Apr 30;12:633910. doi: 10.3389/fimmu.2021.633910. eCollection 2021. Front Immunol. 2021. PMID: 33995353 Free PMC article.
Here, in the context of a clinical trial of cancer patients treated with radiotherapy, we performed longitudinal flow cytometry analyses to identify multiple distinct cell populations in circulating whole blood. ...CD4(+)HLA-DR(+) and NKT-like subse
Here, in the context of a clinical trial of cancer patients treated with radiotherapy, we performed longitudinal flow cytometry
Combined - whole blood and skin fibroblasts- transcriptomic analysis in Psoriatic Arthritis reveals molecular signatures of activity, resistance and early response to treatment.
Grivas A, Grigoriou M, Malissovas N, Sentis G, Filia A, Flouda S, Katsimpri P, Verginis P, Boumpas DT. Grivas A, et al. Front Immunol. 2022 Sep 8;13:964274. doi: 10.3389/fimmu.2022.964274. eCollection 2022. Front Immunol. 2022. PMID: 36159832 Free PMC article.
As control groups, 10 healthy individuals and 10 subjects with rheumatoid arthritis (RA) were recruited combined with public datasets from patients with psoriasis (PsO) and systemic lupus erythematous (SLE). Differential expression analysis and weighted gene co-expression …
As control groups, 10 healthy individuals and 10 subjects with rheumatoid arthritis (RA) were recruited combined with public datasets from p …
Imaging flow cytometry in the assessment of leukocyte-platelet aggregates.
Hui H, Fuller KA, Erber WN, Linden MD. Hui H, et al. Methods. 2017 Jan 1;112:46-54. doi: 10.1016/j.ymeth.2016.10.002. Epub 2016 Oct 5. Methods. 2017. PMID: 27720831
This allows binding of activated platelets to P-Selectin glycoprotein ligand 1 (PSGL-1) expressing leukocytes, forming leukocyte-platelet aggregates (LPAs). Whole blood flow cytometry (FCM) has demonstrated that elevated circulating LPAs (especially …
This allows binding of activated platelets to P-Selectin glycoprotein ligand 1 (PSGL-1) expressing leukocytes, forming leukocyte-platelet ag …
Characterization of Circulating IL-10-Producing Cells in Septic Shock Patients: A Proof of Concept Study.
Fabri A, Kandara K, Coudereau R, Gossez M, Abraham P, Monard C, Cour M, Rimmelé T, Argaud L, Monneret G, Venet F. Fabri A, et al. Front Immunol. 2021 Feb 4;11:615009. doi: 10.3389/fimmu.2020.615009. eCollection 2020. Front Immunol. 2021. PMID: 33613540 Free PMC article.
In this pilot study, we characterized IL-10 producing leukocytes in septic shock patients through whole blood intracellular staining by flow cytometry. ...Using a novel technique for intracellular cytokine measurement in whole blood
In this pilot study, we characterized IL-10 producing leukocytes in septic shock patients through whole blood intracell …
Multidimensional flow cytometry reveals novel platelet subpopulations in response to prostacyclin.
Hindle MS, Spurgeon BEJ, Cheah LT, Webb BA, Naseem KM. Hindle MS, et al. J Thromb Haemost. 2021 Jul;19(7):1800-1812. doi: 10.1111/jth.15330. Epub 2021 Apr 26. J Thromb Haemost. 2021. PMID: 33834609 Free article.
Prostacyclin (PGI(2) ) modulates many aspects of platelet function, but its influence on platelet subpopulations is unknown. OBJECTIVES AND METHODS: We used fluorescent flow cytometry coupled to multidimensional fast Fourier transform-accelerated interpolation-based …
Prostacyclin (PGI(2) ) modulates many aspects of platelet function, but its influence on platelet subpopulations is unknown. OBJECTIVES AND …
Transcriptional correlates of malaria in RTS,S/AS01-vaccinated African children: a matched case-control study.
Moncunill G, Carnes J, Chad Young W, Carpp L, De Rosa S, Campo JJ, Nhabomba A, Mpina M, Jairoce C, Finak G, Haas P, Muriel C, Van P, Sanz H, Dutta S, Mordmüller B, Agnandji ST, Díez-Padrisa N, Williams NA, Aponte JJ, Valim C, Neafsey DE, Daubenberger C, McElrath MJ, Dobaño C, Stuart K, Gottardo R. Moncunill G, et al. Elife. 2022 Jan 21;11:e70393. doi: 10.7554/eLife.70393. Elife. 2022. PMID: 35060479 Free PMC article.
We sought to further understand RTS,S/AS01-induced immune responses associated with vaccine protection. METHODS: Applying the blood transcriptional module (BTM) framework, we characterized the transcriptomic response to RTS,S/AS01 vaccination in antigen-stimulated ( …
We sought to further understand RTS,S/AS01-induced immune responses associated with vaccine protection. METHODS: Applying the blood t …
41 results