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Considerations regarding use of solvents in in vitro cell based assays.
Timm M, Saaby L, Moesby L, Hansen EW. Timm M, et al. Cytotechnology. 2013 Oct;65(5):887-94. doi: 10.1007/s10616-012-9530-6. Epub 2013 Jan 18. Cytotechnology. 2013. PMID: 23328992 Free PMC article.
Since many pharmacological relevant compounds are water-insoluble, solvents are frequently used in cell based assays. Although many reports describe the cellular effects of solvents at high concentrations, only a few relate the effects of sol
Since many pharmacological relevant compounds are water-insoluble, solvents are frequently used in cell based assays
Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation.
Gil-Castell O, Badia JD, Ontoria-Oviedo I, Castellano D, Sepúlveda P, Ribes-Greus A. Gil-Castell O, et al. Mater Sci Eng C Mater Biol Appl. 2020 Feb;107:110296. doi: 10.1016/j.msec.2019.110296. Epub 2019 Oct 14. Mater Sci Eng C Mater Biol Appl. 2020. PMID: 31761169
During in vitro biocompatibility tests, higher cell adhesion and proliferation were found for the 60/40, 50/50 and 40/60 PCL/Ge compositions that was corroborated by MTT assay, fluorescence and microscopy. A weakened structure, more labile to the in vitro
During in vitro biocompatibility tests, higher cell adhesion and proliferation were found for the 60/40, 50/50 and 40/60 PCL/G …
Comparative cytotoxicity of plant protection products and their active ingredients.
Adler-Flindt S, Martin S. Adler-Flindt S, et al. Toxicol In Vitro. 2019 Feb;54:354-366. doi: 10.1016/j.tiv.2018.10.020. Epub 2018 Oct 31. Toxicol In Vitro. 2019. PMID: 30389606 Free article.
However 31% of classified products were not identified revealing a considerable inaccuracy of this method. Based on these results ten PPPs and corresponding ASs were further tested in a cytotoxicity assay employing 3T3 and hFF cells (one PPP and corres …
However 31% of classified products were not identified revealing a considerable inaccuracy of this method. Based on these resu …
Dual-responsive molybdenum disulfide/copper sulfide-based delivery systems for enhanced chemo-photothermal therapy.
Zhang X, Wu J, Williams GR, Yang Y, Niu S, Qian Q, Zhu LM. Zhang X, et al. J Colloid Interface Sci. 2019 Mar 15;539:433-441. doi: 10.1016/j.jcis.2018.12.072. Epub 2018 Dec 19. J Colloid Interface Sci. 2019. PMID: 30599399
Molybdenum disulfide (MoS(2))-based drug delivery systems have shown considerable potential in cancer nanomedicines. ...The presence of both MoS(2) and CuS leads to very high photothermal conversion efficiency (higher than with MoS(2) alone). In vitro experim …
Molybdenum disulfide (MoS(2))-based drug delivery systems have shown considerable potential in cancer nanomedicines. ...The pr …
Dendron-Polymer Conjugate Based Cross-Linked Micelles: A Robust and Versatile Nanosystem for Targeted Delivery.
Calik F, Degirmenci A, Eceoglu M, Sanyal A, Sanyal R. Calik F, et al. Bioconjug Chem. 2019 Apr 17;30(4):1087-1097. doi: 10.1021/acs.bioconjchem.9b00027. Epub 2019 Mar 6. Bioconjug Chem. 2019. PMID: 30789707
Importantly, the amount of passive drug release in neutral pH was considerably lower for the cross-linked micellar systems. Furthermore, treatment of MDA-MB-231 breast cancer cells with nontargeted and targeted cross-linked micelles demonstrated higher internalizati …
Importantly, the amount of passive drug release in neutral pH was considerably lower for the cross-linked micellar systems. Furthermo …
Folate-Targeted Polyethylene Glycol-Modified Photosensitizers for Photodynamic Therapy.
Liu Q, Wang J, Li S, Li G, Chen Q, Hong Z. Liu Q, et al. J Pharm Sci. 2019 Jun;108(6):2102-2111. doi: 10.1016/j.xphs.2019.01.014. Epub 2019 Jan 21. J Pharm Sci. 2019. PMID: 30677421
In our former work, the prepared folic acid (FA)-Pyro conjugates showed considerably improved tumor accumulation and photodynamic therapy (PDT) activity in cell- and animal-based studies. ...Therefore, we chose various lengths of PEGs as linkers to optimize t …
In our former work, the prepared folic acid (FA)-Pyro conjugates showed considerably improved tumor accumulation and photodynamic the …
Bio-guided profiling and HPLC-DAD finger printing of Atriplex lasiantha Boiss.
Zohra T, Ovais M, Khalil AT, Qasim M, Ayaz M, Shinwari ZK, Ahmad S, Zahoor M. Zohra T, et al. BMC Complement Altern Med. 2019 Jan 3;19(1):4. doi: 10.1186/s12906-018-2416-1. BMC Complement Altern Med. 2019. PMID: 30606171 Free PMC article.
The current study has been aimed to validate the therapeutic potential of ethnomedicinally significant plant Atriplex lasiantha Boiss. METHODS: The polarity based extraction process was carried out using fourteen solvents to figure out best extraction solvent
The current study has been aimed to validate the therapeutic potential of ethnomedicinally significant plant Atriplex lasiantha Boiss. METHO …
Optically diffracting hydrogels for screening kinase activity in vitro and in cell lysate: impact of material and solution properties.
MacConaghy KI, Chadly DM, Stoykovich MP, Kaar JL. MacConaghy KI, et al. Anal Chem. 2015 Mar 17;87(6):3467-75. doi: 10.1021/acs.analchem.5b00442. Epub 2015 Mar 5. Anal Chem. 2015. PMID: 25714913
Optically diffracting films based on hydrogel-encapsulated crystalline colloidal arrays have considerable utility as sensors for detecting enzymaticphosphorylation and, thus, in screening small molecule modulators of kinases. ...Ultimately, we showed that, by tuning …
Optically diffracting films based on hydrogel-encapsulated crystalline colloidal arrays have considerable utility as sensors f …
Structure-activity considerations and in vitro approaches to assess the genotoxicity of 19 methane-, benzene- and toluenesulfonic acid esters.
Glowienke S, Frieauff W, Allmendinger T, Martus HJ, Suter W, Mueller L. Glowienke S, et al. Mutat Res. 2005 Mar 7;581(1-2):23-34. doi: 10.1016/j.mrgentox.2004.10.004. Epub 2004 Dec 21. Mutat Res. 2005. PMID: 15725602
Sulfonic acid esters are considered as potentially alkylating agents that may exert genotoxic effects in bacterial and mammalian cell systems. One possible source of human exposure stems from drug synthesis when the salt-forming agents methanesulfonic acid, benzenesulfonic …
Sulfonic acid esters are considered as potentially alkylating agents that may exert genotoxic effects in bacterial and mammalian cell
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery.
Hendershot J, Smith AE, Werfel TA. Hendershot J, et al. J Vis Exp. 2019 May 2;(147). doi: 10.3791/59549. J Vis Exp. 2019. PMID: 31107463
Here, we describe the preparation and in vitro physicochemical/biological characterization of polymeric nanoparticles designed for efficient siRNA delivery, particularly to non-hepatic tissues such as tumors. ...Bioactivity of siRNA nanoparticles is measured using l …
Here, we describe the preparation and in vitro physicochemical/biological characterization of polymeric nanoparticles designed …
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