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Expression of properdin in human monocytes.
Schwaeble W, Huemer HP, Möst J, Dierich MP, Ströbel M, Claus C, Reid KB, Ziegler-Heitbrock HW. Schwaeble W, et al. Eur J Biochem. 1994 Feb 1;219(3):759-64. doi: 10.1111/j.1432-1033.1994.tb18555.x. Eur J Biochem. 1994. PMID: 8112326 Free article.
Northern blot analysis of cell lines derived from fibroblasts, B-cells, hepatoma cells, and cells of the monocyte-macrophage lineage revealed properdin expression only in the myelomonocytic cell line HL-60, in the monoblastic cell line U-937 and in the mon …
Northern blot analysis of cell lines derived from fibroblasts, B-cells, hepatoma cells, and cells of the monocyte-macrophage lineage …
Protective role for properdin in progression of experimental murine atherosclerosis.
Steiner T, Francescut L, Byrne S, Hughes T, Jayanthi A, Guschina I, Harwood J, Cianflone K, Stover C, Francis S. Steiner T, et al. PLoS One. 2014 Mar 25;9(3):e92404. doi: 10.1371/journal.pone.0092404. eCollection 2014. PLoS One. 2014. PMID: 24667818 Free PMC article.
Atherosclerosis was assessed after feeding low fat (LFD) or high fat (HFD) Western type diets to newly generated LDLR(-/-) Properdin(KO) (LDLR(-/-)P(KO)) and LDLR-/-PWT mice. Lipids, lymphocytes and monocytes were similar among genotypes, genders and diets. ...All m …
Atherosclerosis was assessed after feeding low fat (LFD) or high fat (HFD) Western type diets to newly generated LDLR(-/-) Properdin( …
Expression of properdin in complete and incomplete deficiency: normal in vitro synthesis by monocytes in two cases with properdin deficiency type II due to distinct mutations.
Fredrikson GN, Gullstrand B, Westberg J, Sjöholm AG, Uhlén M, Truedsson L. Fredrikson GN, et al. J Clin Immunol. 1998 Jul;18(4):272-82. doi: 10.1023/a:1027385806871. J Clin Immunol. 1998. PMID: 9710744
In type I deficiency no intracellular or secreted properdin was found, indicating rapid intracellular degradation. Monocytes from the males with type II deficiency expressed and secreted properdin normally. ...Analysis of properdin expression …
In type I deficiency no intracellular or secreted properdin was found, indicating rapid intracellular degradation. Monocytes f …
Expression of the components and regulatory proteins of the alternative complement pathway and the membrane attack complex in normal and diseased synovium.
Guc D, Gulati P, Lemercier C, Lappin D, Birnie GD, Whaley K. Guc D, et al. Rheumatol Int. 1993;13(4):139-46. doi: 10.1007/BF00301260. Rheumatol Int. 1993. PMID: 8310205
Monocytes secreted properdin, C3, and factor H but not factor B, factor I, C5, C6, C7, C8 or C9. Fibroblasts and endothelial cells secreted factor B, factor H and factor I, but not properdin, C5, C6, C7, C8 or C9. Lymphocytes did not secrete any of these comp …
Monocytes secreted properdin, C3, and factor H but not factor B, factor I, C5, C6, C7, C8 or C9. Fibroblasts and endothelial c …
Age-related macular degeneration associated polymorphism rs10490924 in ARMS2 results in deficiency of a complement activator.
Micklisch S, Lin Y, Jacob S, Karlstetter M, Dannhausen K, Dasari P, von der Heide M, Dahse HM, Schmölz L, Grassmann F, Alene M, Fauser S, Neumann H, Lorkowski S, Pauly D, Weber BH, Joussen AM, Langmann T, Zipfel PF, Skerka C. Micklisch S, et al. J Neuroinflammation. 2017 Jan 5;14(1):4. doi: 10.1186/s12974-016-0776-3. J Neuroinflammation. 2017. PMID: 28086806 Free PMC article.
Biolayer interferometry defined protein interactions. Furthermore, endogenous ARMS2 gene expression was studied in human blood derived monocytes and in human induced pluripotent stem cell-derived microglia (iPSdM) by PCR and LSM. ...We also demo …
Biolayer interferometry defined protein interactions. Furthermore, endogenous ARMS2 gene expression was studied in human …
Human monocyte-derived dendritic cells are a source of several complement proteins.
Reis ES, Barbuto JA, Isaac L. Reis ES, et al. Inflamm Res. 2006 May;55(5):179-84. doi: 10.1007/s00011-006-0068-y. Inflamm Res. 2006. PMID: 16830104
METHODS: Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot were used to evaluate the expression and production of several complement proteins. RESULTS: We show that DCs produce C3, C5, C9, Factor (F)I, FH, FB, FD and properdin at levels simil …
METHODS: Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot were used to evaluate the expression and productio …
Properdin, a positive regulator of complement activation, is released from secondary granules of stimulated peripheral blood neutrophils.
Wirthmueller U, Dewald B, Thelen M, Schäfer MK, Stover C, Whaley K, North J, Eggleton P, Reid KB, Schwaeble WJ. Wirthmueller U, et al. J Immunol. 1997 May 1;158(9):4444-51. J Immunol. 1997. PMID: 9127010
In contrast to most other components of complement, biosynthesis of properdin is restricted to a few cell types only, i.e., monocytes/macrophages and peripheral blood T cells. This report demonstrates the presence of properdin mRNA in peripheral blood granulo …
In contrast to most other components of complement, biosynthesis of properdin is restricted to a few cell types only, i.e., monocy …
Complement activation by CpG in a human whole blood loop system: mechanisms and immunomodulatory effects.
Mangsbo SM, Sanchez J, Anger K, Lambris JD, Ekdahl KN, Loskog AS, Nilsson B, Tötterman TH. Mangsbo SM, et al. J Immunol. 2009 Nov 15;183(10):6724-32. doi: 10.4049/jimmunol.0902374. Epub 2009 Oct 28. J Immunol. 2009. PMID: 19864604 Free PMC article.
In a human blood loop system, both backbone and sequence-specific effects by CpG were counteracted by selective inhibition of C3. Furthermore, DNA backbone-mediated CD40 and CD83 expression on monocytes and sequence-specific IL-6 and TNF production were reduc …
In a human blood loop system, both backbone and sequence-specific effects by CpG were counteracted by selective inhibition of C3. Fur …
Quantitative In-Depth Transcriptome Analysis Implicates Peritoneal Macrophages as Important Players in the Complement and Coagulation Systems.
Paivandy A, Akula S, Lara S, Fu Z, Olsson AK, Kleinau S, Pejler G, Hellman L. Paivandy A, et al. Int J Mol Sci. 2022 Jan 21;23(3):1185. doi: 10.3390/ijms23031185. Int J Mol Sci. 2022. PMID: 35163105 Free PMC article.
For comparison, we analyzed human peripheral blood monocytes. We show that the human monocytes shared many characteristics with the mouse peritoneal Mphis but that there were also many major differences. Similar to the mouse peritoneal Mphis, the most …
For comparison, we analyzed human peripheral blood monocytes. We show that the human monocytes shared many chara …
Complement alternative pathway acts as a positive feedback amplification of neutrophil activation.
Camous L, Roumenina L, Bigot S, Brachemi S, Frémeaux-Bacchi V, Lesavre P, Halbwachs-Mecarelli L. Camous L, et al. Blood. 2011 Jan 27;117(4):1340-9. doi: 10.1182/blood-2010-05-283564. Epub 2010 Nov 9. Blood. 2011. PMID: 21063021 Free article.
This occurred independently of neutrophil proteases, oxidants, or apoptosis. Neutrophil-secreted properdin was detected on the cell surface and could focus "in situ" the alternative pathway activation. Importantly, complement, in turn, led to further activation of neutroph …
This occurred independently of neutrophil proteases, oxidants, or apoptosis. Neutrophil-secreted properdin was detected on the cell s …