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Human Monocyte Heterogeneity as Revealed by High-Dimensional Mass Cytometry.
Hamers AAJ, Dinh HQ, Thomas GD, Marcovecchio P, Blatchley A, Nakao CS, Kim C, McSkimming C, Taylor AM, Nguyen AT, McNamara CA, Hedrick CC. Hamers AAJ, et al. Arterioscler Thromb Vasc Biol. 2019 Jan;39(1):25-36. doi: 10.1161/ATVBAHA.118.311022. Arterioscler Thromb Vasc Biol. 2019. PMID: 30580568 Free PMC article.
We hypothesized that monocytes were likely more heterogeneous in composition. Approach and Results- We used the high dimensionality of mass cytometry together with the FlowSOM clustering algorithm to accurately identify and define mono …
We hypothesized that monocytes were likely more heterogeneous in composition. Approach and Results- We used the high …
Single-Cell Mapping of Human Brain Cancer Reveals Tumor-Specific Instruction of Tissue-Invading Leukocytes.
Friebel E, Kapolou K, Unger S, Núñez NG, Utz S, Rushing EJ, Regli L, Weller M, Greter M, Tugues S, Neidert MC, Becher B. Friebel E, et al. Cell. 2020 Jun 25;181(7):1626-1642.e20. doi: 10.1016/j.cell.2020.04.055. Epub 2020 May 28. Cell. 2020. PMID: 32470397 Free article.
Here, we have mapped the leukocyte landscape of brain tumors using high-dimensional single-cell profiling (CyTOF). The heterogeneous composition of tissue-resident and invading immune cells within the TME alone permitted a clear distinction between gliomas an …
Here, we have mapped the leukocyte landscape of brain tumors using high-dimensional single-cell profiling (CyTOF). The hete …
High-Dimensional Analysis Reveals Distinct Endotypes in Patients With Idiopathic Inflammatory Myopathies.
Wilfong EM, Bartkowiak T, Vowell KN, Westlake CS, Irish JM, Kendall PL, Crofford LJ, Bonami RH. Wilfong EM, et al. Front Immunol. 2022 Feb 21;13:756018. doi: 10.3389/fimmu.2022.756018. eCollection 2022. Front Immunol. 2022. PMID: 35371068 Free PMC article.
The idiopathic inflammatory myopathies (IIM) are a rare clinically heterogeneous group of conditions affecting the skin, muscle, joint, and lung in various combinations. ...We collected PBMCs from 17 patients with a clinical diagnosis of inflammatory myositis and character …
The idiopathic inflammatory myopathies (IIM) are a rare clinically heterogeneous group of conditions affecting the skin, muscle, join …
Transcriptomic Analysis and High-dimensional Phenotypic Mapping of Mononuclear Phagocytes in Mesenteric Lymph Nodes Reveal Differences Between Ulcerative Colitis and Crohn's Disease.
Chapuy L, Bsat M, Rubio M, Harvey F, Motta V, Schwenter F, Wassef R, Richard C, Deslandres C, Nguyen BN, Soucy G, Hacohen N, Fritz J, Villani AC, Mehta H, Sarfati M. Chapuy L, et al. J Crohns Colitis. 2020 Mar 13;14(3):393-405. doi: 10.1093/ecco-jcc/jjz156. J Crohns Colitis. 2020. PMID: 31541232 Free PMC article.
Heterogeneity of HLA-DR+SIRPalpha + mononuclear phagocytes [MNPs], including macrophages [Mphi], monocyte-derived [Mono] cells, and dendritic cells [DCs], was reported in gut tissue but not yet investigated in mesenteric lymph nodes [MLNs] of IBD patients. We here c …
Heterogeneity of HLA-DR+SIRPalpha + mononuclear phagocytes [MNPs], including macrophages [Mphi], monocyte-derived [Mono] cells …
Individual liver plasmacytoid dendritic cells are capable of producing IFNα and multiple additional cytokines during chronic HCV infection.
Doyle EH, Rahman A, Aloman C, Klepper AL, El-Shamy A, Eng F, Rocha C, Kim S, Haydel B, Florman SS, Fiel MI, Schiano T, Branch AD. Doyle EH, et al. PLoS Pathog. 2019 Jul 29;15(7):e1007935. doi: 10.1371/journal.ppat.1007935. eCollection 2019 Jul. PLoS Pathog. 2019. PMID: 31356648 Free PMC article.
T cells often produce multiple cytokines/chemokines but until recently technical limitations have precluded tests of polyfunctionality in individual pDCs. Mass cytometry (CyTOF) revealed that liver pDCs are the only LMC that produces detectable amounts of IFN …
T cells often produce multiple cytokines/chemokines but until recently technical limitations have precluded tests of polyfunctionality in in …