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2019 2
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Human Monocyte Heterogeneity as Revealed by High-Dimensional Mass Cytometry.
Hamers AAJ, Dinh HQ, Thomas GD, Marcovecchio P, Blatchley A, Nakao CS, Kim C, McSkimming C, Taylor AM, Nguyen AT, McNamara CA, Hedrick CC. Hamers AAJ, et al. Arterioscler Thromb Vasc Biol. 2019 Jan;39(1):25-36. doi: 10.1161/ATVBAHA.118.311022. Arterioscler Thromb Vasc Biol. 2019. PMID: 30580568 Free PMC article.
We hypothesized that monocytes were likely more heterogeneous in composition. Approach and Results- We used the high dimensionality of mass cytometry together with the FlowSOM clustering algorithm to accurately identify and define mono
We hypothesized that monocytes were likely more heterogeneous in composition. Approach and Results- We used the high
Single-Cell Mapping of Human Brain Cancer Reveals Tumor-Specific Instruction of Tissue-Invading Leukocytes.
Friebel E, Kapolou K, Unger S, Núñez NG, Utz S, Rushing EJ, Regli L, Weller M, Greter M, Tugues S, Neidert MC, Becher B. Friebel E, et al. Cell. 2020 Jun 25;181(7):1626-1642.e20. doi: 10.1016/j.cell.2020.04.055. Epub 2020 May 28. Cell. 2020. PMID: 32470397
Here, we have mapped the leukocyte landscape of brain tumors using high-dimensional single-cell profiling (CyTOF). The heterogeneous composition of tissue-resident and invading immune cells within the TME alone permitted a clear distinction between gliomas an …
Here, we have mapped the leukocyte landscape of brain tumors using high-dimensional single-cell profiling (CyTOF). The hete
Transcriptomic Analysis and High-dimensional Phenotypic Mapping of Mononuclear Phagocytes in Mesenteric Lymph Nodes Reveal Differences Between Ulcerative Colitis and Crohn's Disease.
Chapuy L, Bsat M, Rubio M, Harvey F, Motta V, Schwenter F, Wassef R, Richard C, Deslandres C, Nguyen BN, Soucy G, Hacohen N, Fritz J, Villani AC, Mehta H, Sarfati M. Chapuy L, et al. J Crohns Colitis. 2020 Mar 13;14(3):393-405. doi: 10.1093/ecco-jcc/jjz156. J Crohns Colitis. 2020. PMID: 31541232
Heterogeneity of HLA-DR+SIRPalpha + mononuclear phagocytes [MNPs], including macrophages [Mphi], monocyte-derived [Mono] cells, and dendritic cells [DCs], was reported in gut tissue but not yet investigated in mesenteric lymph nodes [MLNs] of IBD patients. We here c
Heterogeneity of HLA-DR+SIRPalpha + mononuclear phagocytes [MNPs], including macrophages [Mphi], monocyte-derived [Mono] cells
Individual liver plasmacytoid dendritic cells are capable of producing IFNα and multiple additional cytokines during chronic HCV infection.
Doyle EH, Rahman A, Aloman C, Klepper AL, El-Shamy A, Eng F, Rocha C, Kim S, Haydel B, Florman SS, Fiel MI, Schiano T, Branch AD. Doyle EH, et al. PLoS Pathog. 2019 Jul 29;15(7):e1007935. doi: 10.1371/journal.ppat.1007935. eCollection 2019 Jul. PLoS Pathog. 2019. PMID: 31356648 Free PMC article.
T cells often produce multiple cytokines/chemokines but until recently technical limitations have precluded tests of polyfunctionality in individual pDCs. Mass cytometry (CyTOF) revealed that liver pDCs are the only LMC that produces detectable amounts of IFN …
T cells often produce multiple cytokines/chemokines but until recently technical limitations have precluded tests of polyfunctionality in in …