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Massively parallel digital transcriptional profiling of single cells.
Zheng GX, Terry JM, Belgrader P, Ryvkin P, Bent ZW, Wilson R, Ziraldo SB, Wheeler TD, McDermott GP, Zhu J, Gregory MT, Shuga J, Montesclaros L, Underwood JG, Masquelier DA, Nishimura SY, Schnall-Levin M, Wyatt PW, Hindson CM, Bharadwaj R, Wong A, Ness KD, Beppu LW, Deeg HJ, McFarland C, Loeb KR, Valente WJ, Ericson NG, Stevens EA, Radich JP, Mikkelsen TS, Hindson BJ, Bielas JH. Zheng GX, et al. Nat Commun. 2017 Jan 16;8:14049. doi: 10.1038/ncomms14049. Nat Commun. 2017. PMID: 28091601 Free PMC article.
Cell encapsulation, of up to 8 samples at a time, takes place in ∼6 min, with ∼50% cell capture efficiency. To demonstrate the system's technical performance, we collected transcriptome data from ∼250k single cells across 29 samples. We validate
Cell encapsulation, of up to 8 samples at a time, takes place in ∼6 min, with ∼50% cell capture efficiency. To demonstrate the
A comparison of massively parallel nucleotide sequencing with oligonucleotide microarrays for global transcription profiling.
Bradford JR, Hey Y, Yates T, Li Y, Pepper SD, Miller CJ. Bradford JR, et al. BMC Genomics. 2010 May 5;11:282. doi: 10.1186/1471-2164-11-282. BMC Genomics. 2010. PMID: 20444259 Free PMC article.
BACKGROUND: RNA-Seq exploits the rapid generation of gigabases of sequence data by Massively Parallel Nucleotide Sequencing, allowing for the mapping and digital quantification of whole transcriptomes. ...Using RNA samples from a normal human breast epithelia …
BACKGROUND: RNA-Seq exploits the rapid generation of gigabases of sequence data by Massively Parallel Nucleotide Sequencing, a …
Using RNA-Seq for gene identification, polymorphism detection and transcript profiling in two alfalfa genotypes with divergent cell wall composition in stems.
Yang SS, Tu ZJ, Cheung F, Xu WW, Lamb JF, Jung HJ, Vance CP, Gronwald JW. Yang SS, et al. BMC Genomics. 2011 Apr 19;12:199. doi: 10.1186/1471-2164-12-199. BMC Genomics. 2011. PMID: 21504589 Free PMC article.
The recent advent of RNA-Seq, a massively parallel sequencing method for transcriptome analysis, provides an opportunity to expand the identification of alfalfa genes and polymorphisms, and conduct in-depth transcript profiling. ...CONCLUSIONS: Our res …
The recent advent of RNA-Seq, a massively parallel sequencing method for transcriptome analysis, provides an opportunity to ex …
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