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Standardization of sampling and sample preparation for analysis of human monocyte subsets in peripheral blood.
Rundgren IM, Bruserud Ø, Ryningen A, Ersvær E. Rundgren IM, et al. J Immunol Methods. 2018 Oct;461:53-62. doi: 10.1016/j.jim.2018.06.003. Epub 2018 Jun 12. J Immunol Methods. 2018. PMID: 29906454
The aim of the present study was to investigate how different pre-analytical parameters influence analysis of monocyte subsets in peripheral blood samples. METHODS: We determined relative levels of monocytes and monocyte
The aim of the present study was to investigate how different pre-analytical parameters influence analysis of monocyte subs
Comparative analysis of different flow cytometry-based immunophenotypic methods for the analysis of CD59 and CD55 expression on major peripheral blood cell subsets.
Hernández-Campo PM, Martín-Ayuso M, Almeida J, López A, Orfao A. Hernández-Campo PM, et al. Cytometry. 2002 Jun 15;50(3):191-201. doi: 10.1002/cyto.10072. Cytometry. 2002. PMID: 12116342 Free article.
METHODS: In the present study, we comparatively analyze the effects of stain-lyse-and-then-wash techniques and lyse-wash-and-then-stain procedures on the detection of both CD55 and CD59 expression on the major peripheral blood (PB) leucocyte subsets, as analy …
METHODS: In the present study, we comparatively analyze the effects of stain-lyse-and-then-wash techniques and lyse-wash-and-then-stain proc …
The effect of erythrocyte lysing reagents on enumeration of leukocyte subpopulations compared with a no-lyse-no-wash protocol.
Plank K, Dorn C, Krause SW. Plank K, et al. Int J Lab Hematol. 2021 Oct;43(5):939-947. doi: 10.1111/ijlh.13484. Epub 2021 Feb 13. Int J Lab Hematol. 2021. PMID: 33580753
In addition, we compared the efficiency of red blood cell (RBC) lysis. RESULTS: Peripheral blood samples from 52 individuals were analyzed. NoL was suitable as reference method, but led to less clear-cut gating of lymphocyte and monocyte
In addition, we compared the efficiency of red blood cell (RBC) lysis. RESULTS: Peripheral blood samples from 52 …
Immunophenotyping As a New Tool for Classification and Monitoring of Systemic Autoimmune Diseases.
Renaudineau Y. Renaudineau Y. Clin Rev Allergy Immunol. 2017 Oct;53(2):177-180. doi: 10.1007/s12016-017-8604-9. Clin Rev Allergy Immunol. 2017. PMID: 28477077
However, immunological changes in mononuclear cells associated with SADs have been only partially described, and usually are limited to analysis of peripheral blood cells (less than 5% of the total mononuclear pool). Another limitation is technological, relat …
However, immunological changes in mononuclear cells associated with SADs have been only partially described, and usually are limited to a
Determination of blood cell subtype concentrations from frozen whole blood samples using TruCount beads.
Langenskiöld C, Mellgren K, Abrahamsson J, Bemark M. Langenskiöld C, et al. Cytometry B Clin Cytom. 2018 Jul;94(4):660-666. doi: 10.1002/cyto.b.21390. Epub 2016 Jul 28. Cytometry B Clin Cytom. 2018. PMID: 27342079 Free article.
RESULTS: The panel can be used on fresh whole-blood samples as well as whole-blood samples that have been frozen after mixing with 10% DMSO. ...This allows for standardized analysis of the samples at a reference laboratory i …
RESULTS: The panel can be used on fresh whole-blood samples as well as whole-blood samples that have been frozen …
Comparing methods for ex vivo characterization of human monocyte phenotypes and in vitro responses.
Johnston L, Harding SA, La Flamme AC. Johnston L, et al. Immunobiology. 2015 Dec;220(12):1305-10. doi: 10.1016/j.imbio.2015.07.014. Epub 2015 Jul 29. Immunobiology. 2015. PMID: 26256247
Furthermore, purification of monocytes using CD14(+) microbeads resulted in a loss of CD14(low)CD16(+) monocytes compared to PBMC samples. Thus, the use of CPT reduced complexity and time compared to Histopaque, and PBMC isolation allowed the analysis
Furthermore, purification of monocytes using CD14(+) microbeads resulted in a loss of CD14(low)CD16(+) monocytes compared to P …