We developed a novel real-time PCR assay for rapid sexing in all three elephant species, which amplifies small fragments of the orthologous sexual chromosome zinc finger protein genes ZFX/ZFY (65 bp). This assay is a simple, inexpensive and reliable tool that is suitable for non-invasive DNA samples and can be incorporated into larger SNP panels for individual identification and population genetic studies.
Keywords: Elephant; KASP probe; Sexing; ZFX/ZFY.
© 2021. Institute of Plant Genetics, Polish Academy of Sciences, Poznan.