Engineering Burkholderia xenovorans LB400 BphA through Site-Directed Mutagenesis at Position 283.
Li J, Min J, Wang Y, Chen W, Kong Y, Guo T, Mahto JK, Sylvestre M, Hu X.
Li J, et al.
Appl Environ Microbiol. 2020 Sep 17;86(19):e01040-20. doi: 10.1128/AEM.01040-20. Print 2020 Sep 17.
Appl Environ Microbiol. 2020.
PMID: 32709719
Free PMC article.
We compared the catalytic reactions of BphAE(LB400) and its variants toward 2,2'-dichlorobiphenyl (2,2'-CB), 2,5-dichlorobiphenyl (2,5-CB), and 2,6-dichlorobiphenyl (2,6-CB). The biochemical data indicate that the Ser283Met substitution alters the orientation …
We compared the catalytic reactions of BphAE(LB400) and its variants toward 2,2'-dichlorobiphenyl (2,2'-CB), 2,5-dichlorobi …