Genotyping of Pulmonary Mycobacterium tuberculosis Isolates from Sudan Using Spoligotyping

Muataz M Eldirdery; Intisar E Alrayah; Mona Oa Awad ElkareIm; Fatima A Khalid; Asrar M A/Salam Elegail; Nuha Y Ibrahim; Eman O M Nour; Rahma H Ali; Elena Hailu; Markos Abebe; Abraham Aseffa; Najem Aldin M Osman; Maowia M Mukhtar; Nihad M A Elhaj; Atif A Elagib

doi:10.12691/ajmr-3-4-1

Genotyping of Pulmonary Mycobacterium tuberculosis Isolates from Sudan Using Spoligotyping

Am J Microbiol Res. 2015;3(4):125-128. doi: 10.12691/ajmr-3-4-1.

Authors

Muataz M Eldirdery¹, Intisar E Alrayah^{1

2}, Mona Oa Awad ElkareIm³, Fatima A Khalid⁴, Asrar M A/Salam Elegail⁵, Nuha Y Ibrahim⁵, Eman O M Nour⁵, Rahma H Ali⁵, Elena Hailu⁶, Markos Abebe⁶, Abraham Aseffa⁶, Najem Aldin M Osman⁷, Maowia M Mukhtar⁸, Nihad M A Elhaj¹, Atif A Elagib¹

Affiliations

¹ Tropical Medicine Research Institute, National Center for Research, Khartoum, Sudan.
² College of Applied Medical Science, Shaqra University, Shaqra, KSA.
³ Blood Transfusion Service, National Blood Transfusion Center, Khartoum, Sudan.
⁴ Tuberculosis Research Center, University of Kassala, Kassala, Sudan.
⁵ National Tuberculosis Reference Laboratory, National Laboratory of Public Health, Khartoum, Sudan.
⁶ Molecular biology Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
⁷ Faculty of Science and Technology, Omdurman Islamic University, Khartoum, Sudan.
⁸ Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.

Abstract

Tuberculosis (TB) remains a major public health problem worldwide due to its high risk of person-to-person transmission, morbidity and mortality [1]. Sudan has a high burden of tuberculosis. Spoligotyping (spacer oligonucleotide typing) a rapid method for genotyping of Mycobacterium tuberculosis using the principle of reverse hybridization. The ecology of the prevalent mycobacteria strain can vary depending on country and region. The aim of this study was to determine the genotyping of Mycobacterium tuberculosis isolated from Sudan using spoligotyping SPOLDB4. A total of 75 Mycobacterium tuberculosis sputum samples were collected from pulmonary Tuberculosis patients attending references Laboratories and diagnostic centers in Khartoum and Eastern Sudan in (2011-2013). The mycobacteria were genotyped using Spoligotyping technique and data obtained were analyzed and compared to the SPOLDB4 database. Among the 75 isolate analyzed, 57(76%) were identified by SPOLDB4 and 18 (24%) could not be matched to any lineages. The most prevalent genotype cluster was MANU2 38 (50.7%) followed by CASI Delhi 8 (10.7%). In the study SIT54 was the most common pattern 37 (49.3%) followed by SIT25 6(8%).

Keywords: Sudan; mycobacterium tuberculosis; spoligotyping.

Grants and funding

D43 TW009127/TW/FIC NIH HHS/United States